Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States

Fred C. Tenover, Linda K. McDougal, Richard V. Goering, George Killgore, Steven J. Projan, Jean B. Patel, Paul M. Dunman

Research output: Contribution to journalArticle

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Abstract

A highly stable strain of Staphylococcus aureus with a pulsed-field gel electrophoresis type of USA300 and multilocus sequence type 8 has been isolated from patients residing in diverse geographic regions of the United States. This strain, designated USA300-0114, is a major cause of skin and soft tissue infections among persons in community settings, including day care centers and correctional facilities, and among sports teams, Native Americans, men who have sex with men, and military recruits. The organism is typically resistant to penicillin, oxacillin, and erythromycin (the latter mediated by msrA) and carries SCCmec type IVa. This strain is variably resistant to tetracycline [mediated by tet(K)]; several recent isolates have decreased susceptibility to fluoroquinolones. S. aureus USA300-0114 harbors the genes encoding the Panton-Valentine leucocidin toxin. DNA sequence analysis of the direct repeat units within the mec determinant of 30 USA300-0114 isolates revealed differences in only a single isolate. Plasmid analysis identified a common 30-kb plasmid that hybridized with blaZ and msrA probes and a 3.1-kb cryptic plasmid. A 4.3-kb plasmid encoding tet(K) and a 2.6-kb plasmid encoding ermC were observed in a few isolates. DNA microarray analysis was used to determine the genetic loci for a series of virulence factors and genes associated with antimicrobial resistance. Comparative genomics between USA300-0114 and three other S. aureus lineages (USA100, USA400, and USA500) defined a set of USA300-0114-specific genes, which may facilitate the strain's pathogenesis within diverse environments.

Original languageEnglish
Pages (from-to)108-118
Number of pages11
JournalJournal of Clinical Microbiology
Volume44
Issue number1
DOIs
StatePublished - Jan 2006

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Methicillin-Resistant Staphylococcus aureus
Plasmids
Staphylococcus aureus
Genes
Oxacillin
Soft Tissue Infections
Genetic Loci
North American Indians
Nucleic Acid Repetitive Sequences
Pulsed Field Gel Electrophoresis
Fluoroquinolones
Virulence Factors
Erythromycin
Microarray Analysis
Genomics
Tetracycline
Oligonucleotide Array Sequence Analysis
DNA Sequence Analysis
Penicillins
Sports

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Microbiology

Cite this

Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States. / Tenover, Fred C.; McDougal, Linda K.; Goering, Richard V.; Killgore, George; Projan, Steven J.; Patel, Jean B.; Dunman, Paul M.

In: Journal of Clinical Microbiology, Vol. 44, No. 1, 01.2006, p. 108-118.

Research output: Contribution to journalArticle

Tenover, Fred C. ; McDougal, Linda K. ; Goering, Richard V. ; Killgore, George ; Projan, Steven J. ; Patel, Jean B. ; Dunman, Paul M. / Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States. In: Journal of Clinical Microbiology. 2006 ; Vol. 44, No. 1. pp. 108-118.
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