Characterization of conformation-dependent prion protein epitopes

Hae Eun Kang, Chu Chun Weng, Eri Saijo, Vicki Saylor, Jifeng Bian, Sehun Kim, Laylaa Ramos, Rachel Angers, Katie Langenfeld, Vadim Khaychuk, Carla Calvi, Jason C. Bartz, Nora Hunter, Glenn C. Telling

Research output: Contribution to journal › Article

23 Citations (Scopus)

Abstract

Background: Despite structural reorganization during disease, conformational prion protein epitopes remain undefined. Results: We identify specific amino acids constituting novel conformational monoclonal antibody epitopes. Conclusion: Immunoreactivities of globular domain epitopes depend on maintenance of regional tertiary structure. Significance: Our studies address how denatured conformational epitopes remain functional, provide insights into normal and disease-related prion protein, and expand epitope tagging options.

Original languageEnglish
Pages (from-to)37219-37232
Number of pages14
JournalJournal of Biological Chemistry
Volume287
Issue number44
DOIs
StatePublished - Oct 26 2012

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Conformations
Epitopes
Prion Diseases
Monoclonal Antibodies
Maintenance
Prions
Prion Proteins
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Kang, H. E., Weng, C. C., Saijo, E., Saylor, V., Bian, J., Kim, S., ... Telling, G. C. (2012). Characterization of conformation-dependent prion protein epitopes. Journal of Biological Chemistry, 287(44), 37219-37232. https://doi.org/10.1074/jbc.M112.395921

Characterization of conformation-dependent prion protein epitopes. / Kang, Hae Eun; Weng, Chu Chun; Saijo, Eri; Saylor, Vicki; Bian, Jifeng; Kim, Sehun; Ramos, Laylaa; Angers, Rachel; Langenfeld, Katie; Khaychuk, Vadim; Calvi, Carla; Bartz, Jason C.; Hunter, Nora; Telling, Glenn C.

In: Journal of Biological Chemistry, Vol. 287, No. 44, 26.10.2012, p. 37219-37232.

Research output: Contribution to journal › Article

Kang, HE, Weng, CC, Saijo, E, Saylor, V, Bian, J, Kim, S, Ramos, L, Angers, R, Langenfeld, K, Khaychuk, V, Calvi, C, Bartz, JC, Hunter, N & Telling, GC 2012, 'Characterization of conformation-dependent prion protein epitopes', Journal of Biological Chemistry, vol. 287, no. 44, pp. 37219-37232. https://doi.org/10.1074/jbc.M112.395921
Kang HE, Weng CC, Saijo E, Saylor V, Bian J, Kim S et al. Characterization of conformation-dependent prion protein epitopes. Journal of Biological Chemistry. 2012 Oct 26;287(44):37219-37232. https://doi.org/10.1074/jbc.M112.395921
Kang, Hae Eun ; Weng, Chu Chun ; Saijo, Eri ; Saylor, Vicki ; Bian, Jifeng ; Kim, Sehun ; Ramos, Laylaa ; Angers, Rachel ; Langenfeld, Katie ; Khaychuk, Vadim ; Calvi, Carla ; Bartz, Jason C. ; Hunter, Nora ; Telling, Glenn C. / Characterization of conformation-dependent prion protein epitopes. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 44. pp. 37219-37232.
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