Characterization of Epstein-Barr virus-induced lymphoproliferation derived from human peripheral blood mononuclear cells transferred to severe combined immunodeficient mice

Motohiko Okano, Yuichi Taguchi, Hirokazu Nakamine, Samuel J. Pirruccello, Jack R. Davis, Kirk Beisel, Kimberly L. Kleveland, Warren G. Sanger, Renee R. Fordyce, David T. Purtilo

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Mice with severe combined immunodeficiency (SCID) received 6 × 107 fresh human peripheral blood mononuclear cells (PBMC) intraperitoneally from Epstein-Barr virus (EBV)-seropositive and -seronegative donors. B95-8 EBV was inoculated intraperitoneally and intravenously to the mice 6 weeks after transfer of seronegative PBMC. Three of four mice transferred with PBMC from two EBV-seropositive donors and two of four mice from two EBV-seronegative donors inoculated with EBV developed fatal EBV-induced lymphoproliferative disease within 6 to 10 weeks. These tumors were oligoclonal orpolyclonal by cytoplasmic immunoglobulin expression. Furthermore no consistent clonal chromosomal abnormalities were shown. Cell lines established from these tumors showed low cloning efficiency in soft agarose. In addition, latent membrane protein, B-lymphocyte activation antigen (CD23), and cell-adhesion molecules (ICAM-1, CD18) all were expressed in the EBV-positive infiltrating lymphoproliferative lesions in each mouse. These results suggest that lymphoid tumors are comparable to lymphoblastoid cell lines immortalized by EBV and are not malignant lymphomas such as Burkitt's lymphoma. This model may be useful for investigating mechanisms responsible for the growing numbers of lymphoproliferative diseases that are occurring in patients with inherited or acquired immunodeficiencies.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalAmerican Journal of Pathology
Volume137
Issue number3
StatePublished - Sep 1990
Externally publishedYes

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SCID Mice
Human Herpesvirus 4
Blood Cells
Tissue Donors
X-Linked Combined Immunodeficiency Diseases
IgE Receptors
Severe Combined Immunodeficiency
Burkitt Lymphoma
Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Lymphocyte Activation
Tumor Cell Line
Chromosome Aberrations
Sepharose
Immunoglobulins
Organism Cloning
Lymphoma
Neoplasms
Membrane Proteins
B-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Characterization of Epstein-Barr virus-induced lymphoproliferation derived from human peripheral blood mononuclear cells transferred to severe combined immunodeficient mice. / Okano, Motohiko; Taguchi, Yuichi; Nakamine, Hirokazu; Pirruccello, Samuel J.; Davis, Jack R.; Beisel, Kirk; Kleveland, Kimberly L.; Sanger, Warren G.; Fordyce, Renee R.; Purtilo, David T.

In: American Journal of Pathology, Vol. 137, No. 3, 09.1990, p. 517-522.

Research output: Contribution to journalArticle

Okano, M, Taguchi, Y, Nakamine, H, Pirruccello, SJ, Davis, JR, Beisel, K, Kleveland, KL, Sanger, WG, Fordyce, RR & Purtilo, DT 1990, 'Characterization of Epstein-Barr virus-induced lymphoproliferation derived from human peripheral blood mononuclear cells transferred to severe combined immunodeficient mice', American Journal of Pathology, vol. 137, no. 3, pp. 517-522.
Okano, Motohiko ; Taguchi, Yuichi ; Nakamine, Hirokazu ; Pirruccello, Samuel J. ; Davis, Jack R. ; Beisel, Kirk ; Kleveland, Kimberly L. ; Sanger, Warren G. ; Fordyce, Renee R. ; Purtilo, David T. / Characterization of Epstein-Barr virus-induced lymphoproliferation derived from human peripheral blood mononuclear cells transferred to severe combined immunodeficient mice. In: American Journal of Pathology. 1990 ; Vol. 137, No. 3. pp. 517-522.
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