Characterization of Muscarinic Cholinergic Receptors in the Crab Nervous System

Abstract: The selective muscarinic antagonist L‐[³H]‐quinuclidinyl benzilate (L‐[³H]QNB) binds reversibly and with high affinity (K_D= 0.3 nM) to a single population (B_max= 105 fmol/mg protein) of specific sites in nervous tissue of the crab Cancer magister. The binding site is stereoselective; (‐)QNB is over 200 times more potent than (+)QNB as an inhibitor of specific L‐[³H]QNB binding. The muscarinic antagonists scopolamine and atropine are over 10,000 times more potent inhibitors of L‐[³H]QNB binding than the nicotinic antagonists deca‐methonium and d‐tubocurarine. The muscarinic agonists oxotremorine, pilocarpine, arecoline, and carbachol also compete effectively for the L‐[³H]QNB binding site. This pharmacological profile strongly suggests the presence of classical muscarinic receptors in the crab nervous system. These receptors are localized to nervous tissue containing cell bodies and neuropil, whereas specific L‐[³H]QNB binding is low or absent in peripheral nerve, skeletal muscle, and artery.