Characterization of novel antimicrobial peptides from the skins of frogs of the Rana esculenta complex

Mohamed F. Ali, Floyd C. Knoop, Hubert Vaudry, J. Michael Conlon

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Rana esculenta is a hybridogenetic hybrid between Rana ridibunda and Rana lessonae and so is best considered as a complex of interbreeding species rather than a discrete single species. In this study, antimicrobial peptides were isolated from a pooled extract of the skins of specimens of the R. esculenta complex collected in the wild. In addition to several peptides belonging to the brevinin and esculentin families that have been previously isolated from skin secretions of a single specimen of R. esculenta, three newly described members of the brevinin-2 family (brevinin-2Ei, brevinin-2Ej, and brevinin-2Ek) and one member of the temporin family (temporin-1Ec) were purified and characterized. In addition, three structurally related peptides with no sequence similarity with antimicrobial peptides isolated from other species of ranid frogs, that potently and selectively inhibit the growth of the Gram-positive bacterium Escherichia coli (minimal inhibitory concentration (MIC<5μM)), were identified. These peptides show limited amino acid sequence similarity to the homologous exon gene products that encode the N-terminal flanking peptides of preprocaerulein, preproxenopsin, and preprolevitide and so have been termed caerulein precursor-related fragments (CPRF-Ea, CPRF-Eb, and CPRF-Ec). The data suggest that there may be considerable polymorphism among specimens from different populations of the R. esculenta complex. It is proposed that the distribution and amino acid sequences of skin antimicrobial peptides may be useful markers for taxonomic classification of particular sub-populations and for an understanding of phylogenetic interrelationships.

Original languageEnglish (US)
Pages (from-to)955-961
Number of pages7
JournalPeptides
Volume24
Issue number7
DOIs
StatePublished - Jul 1 2003

Fingerprint

Rana esculenta
Anura
Skin
Peptides
Amino Acid Sequence
Rana ridibunda
Ranidae
Ceruletide
Amino Acids
Gram-Positive Bacteria
Polymorphism
Escherichia coli
Population
Exons
Bacteria
Genes
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Characterization of novel antimicrobial peptides from the skins of frogs of the Rana esculenta complex. / Ali, Mohamed F.; Knoop, Floyd C.; Vaudry, Hubert; Conlon, J. Michael.

In: Peptides, Vol. 24, No. 7, 01.07.2003, p. 955-961.

Research output: Contribution to journalArticle

Ali, Mohamed F. ; Knoop, Floyd C. ; Vaudry, Hubert ; Conlon, J. Michael. / Characterization of novel antimicrobial peptides from the skins of frogs of the Rana esculenta complex. In: Peptides. 2003 ; Vol. 24, No. 7. pp. 955-961.
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