Characterization of the allelic differences in the mouse cardiac α-myosin heavy chain coding sequence

Brigid K. Quinn-Laquer, Jane E. Kennedy, S. Jack Wei, Kirk Beisel

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have obtained and sequenced the coding sequence of the mouse cardiac α-myosin heavy chain (Myhcα) from the A J, BALB cByJ, C57BL 6J, and DBA 2J inbred mouse strains. Overlapping cDNA sequences were obtained using RNA-PCR and anchor-PCR techniques for these studies. In the A J mouse strain, the full-length message is 5989 bp long and encodes for a protein consisting of 1938 amino acids (Mr 223,689). The protein deduced sequence of the A J Myhcα was compared with corresponding sequences of human and rat Myhcα and β. These results demonstrated that the mouse Myhcα is highly conserved and has maintained the α-isoform-specific divergent cluster observed in other Myhcα proteins. One difference was the loss of a glutamine at residue 1932, which is due to a change in an RNA splicing site sequence. Allelic variability was observed in both nucleotide and amino acid sequences among the four different inbred mouse strains and generally appears to be random in nature. Three of the nucleotide changes resulted in a different amino acid, while the remaining 46 were silent substitutions.

Original languageEnglish
Pages (from-to)176-188
Number of pages13
JournalGenomics
Volume13
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Cardiac Myosins
Myosin Heavy Chains
Inbred Strains Mice
Nucleotides
RNA Splicing
Amino Acids
Polymerase Chain Reaction
Proteins
Glutamine
Amino Acid Sequence
Protein Isoforms
Complementary DNA
RNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Characterization of the allelic differences in the mouse cardiac α-myosin heavy chain coding sequence. / Quinn-Laquer, Brigid K.; Kennedy, Jane E.; Wei, S. Jack; Beisel, Kirk.

In: Genomics, Vol. 13, No. 1, 1992, p. 176-188.

Research output: Contribution to journalArticle

Quinn-Laquer, Brigid K. ; Kennedy, Jane E. ; Wei, S. Jack ; Beisel, Kirk. / Characterization of the allelic differences in the mouse cardiac α-myosin heavy chain coding sequence. In: Genomics. 1992 ; Vol. 13, No. 1. pp. 176-188.
@article{43f52174f3ce45dba4905acc45d62f33,
title = "Characterization of the allelic differences in the mouse cardiac α-myosin heavy chain coding sequence",
abstract = "We have obtained and sequenced the coding sequence of the mouse cardiac α-myosin heavy chain (Myhcα) from the A J, BALB cByJ, C57BL 6J, and DBA 2J inbred mouse strains. Overlapping cDNA sequences were obtained using RNA-PCR and anchor-PCR techniques for these studies. In the A J mouse strain, the full-length message is 5989 bp long and encodes for a protein consisting of 1938 amino acids (Mr 223,689). The protein deduced sequence of the A J Myhcα was compared with corresponding sequences of human and rat Myhcα and β. These results demonstrated that the mouse Myhcα is highly conserved and has maintained the α-isoform-specific divergent cluster observed in other Myhcα proteins. One difference was the loss of a glutamine at residue 1932, which is due to a change in an RNA splicing site sequence. Allelic variability was observed in both nucleotide and amino acid sequences among the four different inbred mouse strains and generally appears to be random in nature. Three of the nucleotide changes resulted in a different amino acid, while the remaining 46 were silent substitutions.",
author = "Quinn-Laquer, {Brigid K.} and Kennedy, {Jane E.} and Wei, {S. Jack} and Kirk Beisel",
year = "1992",
doi = "10.1016/0888-7543(92)90218-H",
language = "English",
volume = "13",
pages = "176--188",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Characterization of the allelic differences in the mouse cardiac α-myosin heavy chain coding sequence

AU - Quinn-Laquer, Brigid K.

AU - Kennedy, Jane E.

AU - Wei, S. Jack

AU - Beisel, Kirk

PY - 1992

Y1 - 1992

N2 - We have obtained and sequenced the coding sequence of the mouse cardiac α-myosin heavy chain (Myhcα) from the A J, BALB cByJ, C57BL 6J, and DBA 2J inbred mouse strains. Overlapping cDNA sequences were obtained using RNA-PCR and anchor-PCR techniques for these studies. In the A J mouse strain, the full-length message is 5989 bp long and encodes for a protein consisting of 1938 amino acids (Mr 223,689). The protein deduced sequence of the A J Myhcα was compared with corresponding sequences of human and rat Myhcα and β. These results demonstrated that the mouse Myhcα is highly conserved and has maintained the α-isoform-specific divergent cluster observed in other Myhcα proteins. One difference was the loss of a glutamine at residue 1932, which is due to a change in an RNA splicing site sequence. Allelic variability was observed in both nucleotide and amino acid sequences among the four different inbred mouse strains and generally appears to be random in nature. Three of the nucleotide changes resulted in a different amino acid, while the remaining 46 were silent substitutions.

AB - We have obtained and sequenced the coding sequence of the mouse cardiac α-myosin heavy chain (Myhcα) from the A J, BALB cByJ, C57BL 6J, and DBA 2J inbred mouse strains. Overlapping cDNA sequences were obtained using RNA-PCR and anchor-PCR techniques for these studies. In the A J mouse strain, the full-length message is 5989 bp long and encodes for a protein consisting of 1938 amino acids (Mr 223,689). The protein deduced sequence of the A J Myhcα was compared with corresponding sequences of human and rat Myhcα and β. These results demonstrated that the mouse Myhcα is highly conserved and has maintained the α-isoform-specific divergent cluster observed in other Myhcα proteins. One difference was the loss of a glutamine at residue 1932, which is due to a change in an RNA splicing site sequence. Allelic variability was observed in both nucleotide and amino acid sequences among the four different inbred mouse strains and generally appears to be random in nature. Three of the nucleotide changes resulted in a different amino acid, while the remaining 46 were silent substitutions.

UR - http://www.scopus.com/inward/record.url?scp=0026580703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026580703&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(92)90218-H

DO - 10.1016/0888-7543(92)90218-H

M3 - Article

C2 - 1577481

AN - SCOPUS:0026580703

VL - 13

SP - 176

EP - 188

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 1

ER -