Abstract
We have obtained and sequenced the coding sequence of the mouse cardiac α-myosin heavy chain (Myhcα) from the A J, BALB cByJ, C57BL 6J, and DBA 2J inbred mouse strains. Overlapping cDNA sequences were obtained using RNA-PCR and anchor-PCR techniques for these studies. In the A J mouse strain, the full-length message is 5989 bp long and encodes for a protein consisting of 1938 amino acids (Mr 223,689). The protein deduced sequence of the A J Myhcα was compared with corresponding sequences of human and rat Myhcα and β. These results demonstrated that the mouse Myhcα is highly conserved and has maintained the α-isoform-specific divergent cluster observed in other Myhcα proteins. One difference was the loss of a glutamine at residue 1932, which is due to a change in an RNA splicing site sequence. Allelic variability was observed in both nucleotide and amino acid sequences among the four different inbred mouse strains and generally appears to be random in nature. Three of the nucleotide changes resulted in a different amino acid, while the remaining 46 were silent substitutions.
| Original language | English |
|---|---|
| Pages (from-to) | 176-188 |
| Number of pages | 13 |
| Journal | Genomics |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1992 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Genetics
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Characterization of the allelic differences in the mouse cardiac α-myosin heavy chain coding sequence. / Quinn-Laquer, Brigid K.; Kennedy, Jane E.; Wei, S. Jack; Beisel, Kirk.
In: Genomics, Vol. 13, No. 1, 1992, p. 176-188.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of the allelic differences in the mouse cardiac α-myosin heavy chain coding sequence
AU - Quinn-Laquer, Brigid K.
AU - Kennedy, Jane E.
AU - Wei, S. Jack
AU - Beisel, Kirk
PY - 1992
Y1 - 1992
N2 - We have obtained and sequenced the coding sequence of the mouse cardiac α-myosin heavy chain (Myhcα) from the A J, BALB cByJ, C57BL 6J, and DBA 2J inbred mouse strains. Overlapping cDNA sequences were obtained using RNA-PCR and anchor-PCR techniques for these studies. In the A J mouse strain, the full-length message is 5989 bp long and encodes for a protein consisting of 1938 amino acids (Mr 223,689). The protein deduced sequence of the A J Myhcα was compared with corresponding sequences of human and rat Myhcα and β. These results demonstrated that the mouse Myhcα is highly conserved and has maintained the α-isoform-specific divergent cluster observed in other Myhcα proteins. One difference was the loss of a glutamine at residue 1932, which is due to a change in an RNA splicing site sequence. Allelic variability was observed in both nucleotide and amino acid sequences among the four different inbred mouse strains and generally appears to be random in nature. Three of the nucleotide changes resulted in a different amino acid, while the remaining 46 were silent substitutions.
AB - We have obtained and sequenced the coding sequence of the mouse cardiac α-myosin heavy chain (Myhcα) from the A J, BALB cByJ, C57BL 6J, and DBA 2J inbred mouse strains. Overlapping cDNA sequences were obtained using RNA-PCR and anchor-PCR techniques for these studies. In the A J mouse strain, the full-length message is 5989 bp long and encodes for a protein consisting of 1938 amino acids (Mr 223,689). The protein deduced sequence of the A J Myhcα was compared with corresponding sequences of human and rat Myhcα and β. These results demonstrated that the mouse Myhcα is highly conserved and has maintained the α-isoform-specific divergent cluster observed in other Myhcα proteins. One difference was the loss of a glutamine at residue 1932, which is due to a change in an RNA splicing site sequence. Allelic variability was observed in both nucleotide and amino acid sequences among the four different inbred mouse strains and generally appears to be random in nature. Three of the nucleotide changes resulted in a different amino acid, while the remaining 46 were silent substitutions.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026580703&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(92)90218-H
DO - 10.1016/0888-7543(92)90218-H
M3 - Article
C2 - 1577481
AN - SCOPUS:0026580703
VL - 13
SP - 176
EP - 188
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -