Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick C. Swanson; Gary D. Glick

doi:10.1016/S0960-894X(01)81028-3

Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick C. Swanson, Gary D. Glick

Research output: Contribution to journal › Article

7 Scopus citations

Abstract

Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

Original language	English (US)
Pages (from-to)	2117-2118
Number of pages	2
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	3
Issue number	10
DOIs	https://doi.org/10.1016/S0960-894X(01)81028-3
State	Published - Oct 1993
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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Swanson, P. C., & Glick, G. D. (1993). Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides. Bioorganic and Medicinal Chemistry Letters, 3(10), 2117-2118. https://doi.org/10.1016/S0960-894X(01)81028-3

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