Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick Swanson, Gary D. Glick

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

Original languageEnglish
Pages (from-to)2117-2118
Number of pages2
JournalBioorganic and Medicinal Chemistry Letters
Volume3
Issue number10
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Oligodeoxyribonucleotides
Disulfides
Molecular recognition
Ethylmaleimide
Alkylation
Sulfhydryl Compounds
Crosslinking
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides. / Swanson, Patrick; Glick, Gary D.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 3, No. 10, 1993, p. 2117-2118.

Research output: Contribution to journalArticle

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