Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick C. Swanson; Gary D. Glick

doi:10.1016/S0960-894X(01)81028-3

Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick C. Swanson, Gary D. Glick

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

Original language	English (US)
Pages (from-to)	2117-2118
Number of pages	2
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	3
Issue number	10
DOIs	https://doi.org/10.1016/S0960-894X(01)81028-3
State	Published - Oct 1993
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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abstract = "Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.",

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AU - Glick, Gary D.

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AB - Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

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DO - 10.1016/S0960-894X(01)81028-3

M3 - Article

AN - SCOPUS:0027372199

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 10

ER -

Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Abstract

All Science Journal Classification (ASJC) codes

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