Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick C. Swanson; Gary D. Glick

doi:10.1016/S0960-894X(01)81028-3

Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick C. Swanson, Gary D. Glick

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

Original language	English (US)
Pages (from-to)	2117-2118
Number of pages	2
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	3
Issue number	10
DOIs	https://doi.org/10.1016/S0960-894X(01)81028-3
State	Published - Oct 1993
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/S0960-894X(01)81028-3

Fingerprint Dive into the research topics of 'Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides'. Together they form a unique fingerprint.

Cite this

@article{79b48ac842934d1da2fcf5b76ac232aa,

title = "Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides",

abstract = "Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.",

author = "Swanson, {Patrick C.} and Glick, {Gary D.}",

year = "1993",

month = oct,

doi = "10.1016/S0960-894X(01)81028-3",

language = "English (US)",

volume = "3",

pages = "2117--2118",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Limited",

number = "10",

}

TY - JOUR

T1 - Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

AU - Swanson, Patrick C.

AU - Glick, Gary D.

PY - 1993/10

Y1 - 1993/10

N2 - Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

AB - Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

UR - http://www.scopus.com/inward/record.url?scp=0027372199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027372199&partnerID=8YFLogxK

U2 - 10.1016/S0960-894X(01)81028-3

DO - 10.1016/S0960-894X(01)81028-3

M3 - Article

AN - SCOPUS:0027372199

VL - 3

SP - 2117

EP - 2118

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 10

ER -