Abstract
Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.
Original language | English (US) |
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Pages (from-to) | 2117-2118 |
Number of pages | 2 |
Journal | Bioorganic and Medicinal Chemistry Letters |
Volume | 3 |
Issue number | 10 |
DOIs | |
State | Published - Oct 1993 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry