Abstract
Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.
| Original language | English |
|---|---|
| Pages (from-to) | 2117-2118 |
| Number of pages | 2 |
| Journal | Bioorganic and Medicinal Chemistry Letters |
| Volume | 3 |
| Issue number | 10 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Drug Discovery
- Pharmaceutical Science
Cite this
Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides. / Swanson, Patrick; Glick, Gary D.
In: Bioorganic and Medicinal Chemistry Letters, Vol. 3, No. 10, 1993, p. 2117-2118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides
AU - Swanson, Patrick
AU - Glick, Gary D.
PY - 1993
Y1 - 1993
N2 - Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.
AB - Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.
UR - http://www.scopus.com/inward/record.url?scp=0027372199&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027372199&partnerID=8YFLogxK
U2 - 10.1016/S0960-894X(01)81028-3
DO - 10.1016/S0960-894X(01)81028-3
M3 - Article
AN - SCOPUS:0027372199
VL - 3
SP - 2117
EP - 2118
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
SN - 0960-894X
IS - 10
ER -