Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick Swanson, Gary D. Glick

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

Original languageEnglish
Pages (from-to)2117-2118
Number of pages2
JournalBioorganic and Medicinal Chemistry Letters
Volume3
Issue number10
DOIs
StatePublished - 1993
Externally publishedYes

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Oligodeoxyribonucleotides
Disulfides
Molecular recognition
Ethylmaleimide
Alkylation
Sulfhydryl Compounds
Crosslinking
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides. / Swanson, Patrick; Glick, Gary D.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 3, No. 10, 1993, p. 2117-2118.

Research output: Contribution to journal › Article

Swanson, P & Glick, GD 1993, 'Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides', Bioorganic and Medicinal Chemistry Letters, vol. 3, no. 10, pp. 2117-2118. https://doi.org/10.1016/S0960-894X(01)81028-3
Swanson P, Glick GD. Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides. Bioorganic and Medicinal Chemistry Letters. 1993;3(10):2117-2118. https://doi.org/10.1016/S0960-894X(01)81028-3
Swanson, Patrick ; Glick, Gary D. / Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides. In: Bioorganic and Medicinal Chemistry Letters. 1993 ; Vol. 3, No. 10. pp. 2117-2118.
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