Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides

Patrick C. Swanson, Gary D. Glick

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA stabilized by crosslinking.

Original languageEnglish (US)
Pages (from-to)2117-2118
Number of pages2
JournalBioorganic and Medicinal Chemistry Letters
Volume3
Issue number10
DOIs
StatePublished - Oct 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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