Chick hair cells do not exhibit voltage-dependent somatic motility

David Z. He, Kirk Beisel, Lin Chen, Da Lian Ding, Shuping Jia, Bernd Fritzsch, Richard Salvi

Research output: Contribution to journalReview article

33 Citations (Scopus)

Abstract

It is generally believed that mechanical amplification by cochlear hair cells is necessary to enhance the sensitivity and frequency selectivity of hearing. In the mammalian ear, the basis of cochlear amplification is believed to be the voltage-dependent electromotility of outer hair cells (OHCs). The avian basilar papilla contains tall and short hair cells, with the former being comparable to inner hair cells, and the latter comparable to OHCs, based on their innervation patterns. In this study, we sought evidence for somatic electromotility by direct measurements of voltage-dependent length changes in both tall and short hair cells at nanometre resolution. Microchamber and whole-cell voltage-clamp techniques were used. Motility was measured with a photodiode-based measurement system. Non-linear capacitance, an electrical signature of somatic motility, was also measured to complement motility measurement. Significantly, chick hair cells did not exhibit somatic motility nor express non-linear capacitance. The lack of somatic motility suggests that in avian hair cells the active process resides elsewhere, most likely in the hair cell stereocilia.

Original languageEnglish
Pages (from-to)511-520
Number of pages10
JournalJournal of Physiology
Volume546
Issue number2
DOIs
StatePublished - Jan 15 2003

Fingerprint

Outer Auditory Hair Cells
Inner Auditory Hair Cells
Electric Capacitance
Auditory Hair Cells
Stereocilia
Organ of Corti
Cochlea
Patch-Clamp Techniques
Hearing
Ear

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Chick hair cells do not exhibit voltage-dependent somatic motility. / He, David Z.; Beisel, Kirk; Chen, Lin; Ding, Da Lian; Jia, Shuping; Fritzsch, Bernd; Salvi, Richard.

In: Journal of Physiology, Vol. 546, No. 2, 15.01.2003, p. 511-520.

Research output: Contribution to journalReview article

He, David Z. ; Beisel, Kirk ; Chen, Lin ; Ding, Da Lian ; Jia, Shuping ; Fritzsch, Bernd ; Salvi, Richard. / Chick hair cells do not exhibit voltage-dependent somatic motility. In: Journal of Physiology. 2003 ; Vol. 546, No. 2. pp. 511-520.
@article{d194167d6f45442e8d784e9bc96f2e99,
title = "Chick hair cells do not exhibit voltage-dependent somatic motility",
abstract = "It is generally believed that mechanical amplification by cochlear hair cells is necessary to enhance the sensitivity and frequency selectivity of hearing. In the mammalian ear, the basis of cochlear amplification is believed to be the voltage-dependent electromotility of outer hair cells (OHCs). The avian basilar papilla contains tall and short hair cells, with the former being comparable to inner hair cells, and the latter comparable to OHCs, based on their innervation patterns. In this study, we sought evidence for somatic electromotility by direct measurements of voltage-dependent length changes in both tall and short hair cells at nanometre resolution. Microchamber and whole-cell voltage-clamp techniques were used. Motility was measured with a photodiode-based measurement system. Non-linear capacitance, an electrical signature of somatic motility, was also measured to complement motility measurement. Significantly, chick hair cells did not exhibit somatic motility nor express non-linear capacitance. The lack of somatic motility suggests that in avian hair cells the active process resides elsewhere, most likely in the hair cell stereocilia.",
author = "He, {David Z.} and Kirk Beisel and Lin Chen and Ding, {Da Lian} and Shuping Jia and Bernd Fritzsch and Richard Salvi",
year = "2003",
month = "1",
day = "15",
doi = "10.1113/jphysiol.2002.026070",
language = "English",
volume = "546",
pages = "511--520",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Chick hair cells do not exhibit voltage-dependent somatic motility

AU - He, David Z.

AU - Beisel, Kirk

AU - Chen, Lin

AU - Ding, Da Lian

AU - Jia, Shuping

AU - Fritzsch, Bernd

AU - Salvi, Richard

PY - 2003/1/15

Y1 - 2003/1/15

N2 - It is generally believed that mechanical amplification by cochlear hair cells is necessary to enhance the sensitivity and frequency selectivity of hearing. In the mammalian ear, the basis of cochlear amplification is believed to be the voltage-dependent electromotility of outer hair cells (OHCs). The avian basilar papilla contains tall and short hair cells, with the former being comparable to inner hair cells, and the latter comparable to OHCs, based on their innervation patterns. In this study, we sought evidence for somatic electromotility by direct measurements of voltage-dependent length changes in both tall and short hair cells at nanometre resolution. Microchamber and whole-cell voltage-clamp techniques were used. Motility was measured with a photodiode-based measurement system. Non-linear capacitance, an electrical signature of somatic motility, was also measured to complement motility measurement. Significantly, chick hair cells did not exhibit somatic motility nor express non-linear capacitance. The lack of somatic motility suggests that in avian hair cells the active process resides elsewhere, most likely in the hair cell stereocilia.

AB - It is generally believed that mechanical amplification by cochlear hair cells is necessary to enhance the sensitivity and frequency selectivity of hearing. In the mammalian ear, the basis of cochlear amplification is believed to be the voltage-dependent electromotility of outer hair cells (OHCs). The avian basilar papilla contains tall and short hair cells, with the former being comparable to inner hair cells, and the latter comparable to OHCs, based on their innervation patterns. In this study, we sought evidence for somatic electromotility by direct measurements of voltage-dependent length changes in both tall and short hair cells at nanometre resolution. Microchamber and whole-cell voltage-clamp techniques were used. Motility was measured with a photodiode-based measurement system. Non-linear capacitance, an electrical signature of somatic motility, was also measured to complement motility measurement. Significantly, chick hair cells did not exhibit somatic motility nor express non-linear capacitance. The lack of somatic motility suggests that in avian hair cells the active process resides elsewhere, most likely in the hair cell stereocilia.

UR - http://www.scopus.com/inward/record.url?scp=12244255116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12244255116&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2002.026070

DO - 10.1113/jphysiol.2002.026070

M3 - Review article

VL - 546

SP - 511

EP - 520

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 2

ER -