Abstract
Chlamydia pneumoniae, a respiratory pathogen, has been associated with occlusive vascular disease, including atherosclerosis and intimal hyperplasia, through seroepidemiologic studies. Furthermore, using immunohistochemistiy (IHC), polymerase chain reaction (PCR), transmission electron microscopy (TEM), and in situ hybridization, this association has been reconfirmed by detecting this organism in atherosclerotic vascular tissue. This review summarizes and critically analyzes these findings and also discusses various mechanisms of how Chlamydia pneumoniae could be involved in the pathogenesis of occlusive vascular disease. Although more studies are needed to reproduce these results and, possibly, uncover a mechanism, the current literature fails to include detailed methodologies for studying Chlamydia pneumoniae. Therefore, to provide a general standard, we have also outlined specific protocols for IHC, PCR, and TEM. These protocols incorporate essential components from various studies and are presented in a concise and easily adaptable format.
| Original language | English |
|---|---|
| Pages (from-to) | 51-61 |
| Number of pages | 11 |
| Journal | Journal of Pharmacological and Toxicological Methods |
| Volume | 39 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1998 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Toxicology
Cite this
Chlamydia pneumoniae and occlusive vascular disease : Identification and characterization. / Pai, Jeevan; Knoop, Floyd C.; Hunter, William J.; Agrawal, Devendra K.
In: Journal of Pharmacological and Toxicological Methods, Vol. 39, No. 1, 02.1998, p. 51-61.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chlamydia pneumoniae and occlusive vascular disease
T2 - Identification and characterization
AU - Pai, Jeevan
AU - Knoop, Floyd C.
AU - Hunter, William J.
AU - Agrawal, Devendra K.
PY - 1998/2
Y1 - 1998/2
N2 - Chlamydia pneumoniae, a respiratory pathogen, has been associated with occlusive vascular disease, including atherosclerosis and intimal hyperplasia, through seroepidemiologic studies. Furthermore, using immunohistochemistiy (IHC), polymerase chain reaction (PCR), transmission electron microscopy (TEM), and in situ hybridization, this association has been reconfirmed by detecting this organism in atherosclerotic vascular tissue. This review summarizes and critically analyzes these findings and also discusses various mechanisms of how Chlamydia pneumoniae could be involved in the pathogenesis of occlusive vascular disease. Although more studies are needed to reproduce these results and, possibly, uncover a mechanism, the current literature fails to include detailed methodologies for studying Chlamydia pneumoniae. Therefore, to provide a general standard, we have also outlined specific protocols for IHC, PCR, and TEM. These protocols incorporate essential components from various studies and are presented in a concise and easily adaptable format.
AB - Chlamydia pneumoniae, a respiratory pathogen, has been associated with occlusive vascular disease, including atherosclerosis and intimal hyperplasia, through seroepidemiologic studies. Furthermore, using immunohistochemistiy (IHC), polymerase chain reaction (PCR), transmission electron microscopy (TEM), and in situ hybridization, this association has been reconfirmed by detecting this organism in atherosclerotic vascular tissue. This review summarizes and critically analyzes these findings and also discusses various mechanisms of how Chlamydia pneumoniae could be involved in the pathogenesis of occlusive vascular disease. Although more studies are needed to reproduce these results and, possibly, uncover a mechanism, the current literature fails to include detailed methodologies for studying Chlamydia pneumoniae. Therefore, to provide a general standard, we have also outlined specific protocols for IHC, PCR, and TEM. These protocols incorporate essential components from various studies and are presented in a concise and easily adaptable format.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031947949&partnerID=8YFLogxK
U2 - 10.1016/S1056-8719(98)00002-1
DO - 10.1016/S1056-8719(98)00002-1
M3 - Article
C2 - 9596148
AN - SCOPUS:0031947949
VL - 39
SP - 51
EP - 61
JO - Journal of Pharmacological and Toxicological Methods
JF - Journal of Pharmacological and Toxicological Methods
SN - 1056-8719
IS - 1
ER -