Abstract
Chronic treatment of rats with theophylline (75 mg/kg twice-daily for 21 consecutive days) significantly increased the specific binding of [3HCHA and [3H]DPCPX in cerebral cortical membranes. The absolute increase in the number of binding sites following theophylline treatment was approximately the same for each ligand, although this number represents a larger percentage of the total sites available to [3H]CHA. Saturation analysis of [3H]DPCPX binding indicated that theophylline treatment increased the maximum number of binding sites from 799 ± 13 to 920 ± 22 fmol/mg protein, while the affinity of [3H]DPCPX for A1 receptors was unaltered.These results suggest that chronic theophylline exposure produces both an increase in the number of A1 adenosine receptors and an enhancement of coupling of these receptors to G proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 757-760 |
| Number of pages | 4 |
| Journal | Neuropharmacology |
| Volume | 27 |
| Issue number | 7 |
| DOIs | |
| State | Published - 1988 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Cellular and Molecular Neuroscience
- Drug Discovery
- Pharmacology
Cite this
Chronic theophylline exposure increases agonist and antagonist binding to A1 adenosine receptors in rat brain. / Sanders, R. C.; Murray, Thomas F.
In: Neuropharmacology, Vol. 27, No. 7, 1988, p. 757-760.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chronic theophylline exposure increases agonist and antagonist binding to A1 adenosine receptors in rat brain
AU - Sanders, R. C.
AU - Murray, Thomas F.
PY - 1988
Y1 - 1988
N2 - Chronic treatment of rats with theophylline (75 mg/kg twice-daily for 21 consecutive days) significantly increased the specific binding of [3HCHA and [3H]DPCPX in cerebral cortical membranes. The absolute increase in the number of binding sites following theophylline treatment was approximately the same for each ligand, although this number represents a larger percentage of the total sites available to [3H]CHA. Saturation analysis of [3H]DPCPX binding indicated that theophylline treatment increased the maximum number of binding sites from 799 ± 13 to 920 ± 22 fmol/mg protein, while the affinity of [3H]DPCPX for A1 receptors was unaltered.These results suggest that chronic theophylline exposure produces both an increase in the number of A1 adenosine receptors and an enhancement of coupling of these receptors to G proteins.
AB - Chronic treatment of rats with theophylline (75 mg/kg twice-daily for 21 consecutive days) significantly increased the specific binding of [3HCHA and [3H]DPCPX in cerebral cortical membranes. The absolute increase in the number of binding sites following theophylline treatment was approximately the same for each ligand, although this number represents a larger percentage of the total sites available to [3H]CHA. Saturation analysis of [3H]DPCPX binding indicated that theophylline treatment increased the maximum number of binding sites from 799 ± 13 to 920 ± 22 fmol/mg protein, while the affinity of [3H]DPCPX for A1 receptors was unaltered.These results suggest that chronic theophylline exposure produces both an increase in the number of A1 adenosine receptors and an enhancement of coupling of these receptors to G proteins.
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U2 - 10.1016/0028-3908(88)90086-X
DO - 10.1016/0028-3908(88)90086-X
M3 - Article
VL - 27
SP - 757
EP - 760
JO - Neuropharmacology
JF - Neuropharmacology
SN - 0028-3908
IS - 7
ER -