Cigarette smoke-induced differential expression of the genes involved in exocrine function of the rat pancreas

Uwe A. Wittel, Ajay P. Singh, Brandon J. Henley, Mahefatiana Andrianifahanana, Mohammed P. Akhter, Diane M. Cullen, Surinder K. Batra

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

OBJECTIVE: Little is known about the molecular and biological aspects of the epidemiological association between smoking and pancreatic pathology, such as chronic pancreatitis and pancreatic cancer. Recently, we reported that tobacco smoke exposure induced morphological alterations in the rat pancreas. Here, we have investigated the alterations in the expression of genes associated with exocrine pancreatic function and cellular differentiation upon exposure to cigarette smoke. METHODS: Female rats were exposed to environmental smoke inhalation for 2 d/wk (70 min/d) for 12 weeks. The expression profiles of trypsinogen, pancreas-specific trypsin inhibitor, cholecystokinin A receptor, cystic fibrosis transmembrane conductance regulator (CFTR), carbonic anhydrase, and Muc1 and Muc4 mucins transcripts were analyzed by RNA slot blot analysis. Muc4 expression was also examined by immunohistochemistry. RESULTS: Our data revealed that the ratio of trypsinogen to that of the protective pancreas-specific trypsin inhibitor was elevated upon cigarette smoke exposure. The expression of carbonic anhydrase and CFTR remained unaltered when inflammatory signs were not detected in histological examinations. On the other hand, when pancreatic inflammation was present, the levels of CFTR and carbonic anhydrase were increased, indicating ductal and/or centroacinar cell involvement. No changes in the expression of Muc1 and Muc4 mucins were observed. CONCLUSIONS: Our data show that cigarette smoke exposure leads to an increased vulnerability to pancreatic self-digestion. Moreover, the concomitant involvement of pancreatic ducts occurs only when focal pancreatic inflammation is present.

Original languageEnglish
Pages (from-to)364-370
Number of pages7
JournalPancreas
Volume33
Issue number4
DOIs
StatePublished - Nov 2006

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Smoke
Tobacco Products
Pancreas
Cystic Fibrosis Transmembrane Conductance Regulator
Carbonic Anhydrases
Gene Expression
Trypsinogen
Mucin-1
Trypsin Inhibitors
Cholecystokinin A Receptor
Inflammation
Pancreatic Ducts
Chronic Pancreatitis
Pancreatic Neoplasms
Inhalation
Tobacco
Digestion
Smoking
Immunohistochemistry
RNA

All Science Journal Classification (ASJC) codes

  • Gastroenterology
  • Endocrinology

Cite this

Wittel, U. A., Singh, A. P., Henley, B. J., Andrianifahanana, M., Akhter, M. P., Cullen, D. M., & Batra, S. K. (2006). Cigarette smoke-induced differential expression of the genes involved in exocrine function of the rat pancreas. Pancreas, 33(4), 364-370. https://doi.org/10.1097/01.mpa.0000240601.80570.31

Cigarette smoke-induced differential expression of the genes involved in exocrine function of the rat pancreas. / Wittel, Uwe A.; Singh, Ajay P.; Henley, Brandon J.; Andrianifahanana, Mahefatiana; Akhter, Mohammed P.; Cullen, Diane M.; Batra, Surinder K.

In: Pancreas, Vol. 33, No. 4, 11.2006, p. 364-370.

Research output: Contribution to journalArticle

Wittel, UA, Singh, AP, Henley, BJ, Andrianifahanana, M, Akhter, MP, Cullen, DM & Batra, SK 2006, 'Cigarette smoke-induced differential expression of the genes involved in exocrine function of the rat pancreas', Pancreas, vol. 33, no. 4, pp. 364-370. https://doi.org/10.1097/01.mpa.0000240601.80570.31
Wittel, Uwe A. ; Singh, Ajay P. ; Henley, Brandon J. ; Andrianifahanana, Mahefatiana ; Akhter, Mohammed P. ; Cullen, Diane M. ; Batra, Surinder K. / Cigarette smoke-induced differential expression of the genes involved in exocrine function of the rat pancreas. In: Pancreas. 2006 ; Vol. 33, No. 4. pp. 364-370.
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