Cl- channels are expressed in human normal monocytes

A functional role in migration, adhesion and volume change

M. J. Kim, G. Cheng, Devendra K. Agrawal

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Increased adhesion and diapedesis of monocytes appear to be primary initiating factors in the pathophysiology of occlusive vascular diseases, including atherosclerosis and restenosis. However, the underlying mechanisms of transendothelial migration and invasion of monocytes into the blood vessels are not known. Alterations in ion channels on the cell membrane are generally involved in induced changes in shape and volume. In the present study, we investigated the expression and functional role of chloride channels in freshly isolated human blood monocytes. The Cl- currents in whole-cells were measured by the patch-clamp technique. We observed whole cell Cl- currents, which were time-independent and outwardly rectifying. The chloride channel blockers 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and R(+)-[(6,7-dichloro-2-cyclopentyl-2,3-dihydro-2-methyl-1-oxo-1H-inden-5yl)-oxy] acetic acid 94 (IAA94) attenuated the Cl- currents. NPPB and IAA94 also inhibited chemotaxis of monocytes, as measured in Boyden chemotactic chambers, with the same sensitivity. NPPB but not IAA94, increased the cell volume as measured by shape change, and decreased tumour necrosis factor (TNF)-α-induced monocyte adhesion to endothelial cells. These results suggest that monocytes contain Cl- channels which regulate transendothelial migration of monocytes, due presumably to an alteration in cell volume.

Original languageEnglish
Pages (from-to)453-459
Number of pages7
JournalClinical and Experimental Immunology
Volume138
Issue number3
DOIs
StatePublished - Dec 2004

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Monocytes
Transendothelial and Transepithelial Migration
Benzoic Acid
Cell Size
Chloride Channels
Patch-Clamp Techniques
Chemotaxis
Ion Channels
Vascular Diseases
Acetic Acid
Blood Vessels
Atherosclerosis
Endothelial Cells
Tumor Necrosis Factor-alpha
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Cl- channels are expressed in human normal monocytes : A functional role in migration, adhesion and volume change. / Kim, M. J.; Cheng, G.; Agrawal, Devendra K.

In: Clinical and Experimental Immunology, Vol. 138, No. 3, 12.2004, p. 453-459.

Research output: Contribution to journalArticle

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