Cochlear whole mount in situ hybridization

Identification of longitudinal and radial gradients

Tiffany N. Judice, Nathan C. Nelson, Chase L. Beisel, Duane C. Delimont, Bernd Fritzsch, Kirk Beisel

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The morphology of the organ of Corti has a radial asymmetry and also changes longitudinally from base to apex. Cellular localization of transcripts within the inner ear has relied primarily on the use of sectioned tissue with in situ hybridization. However, radial and longitudinal gradients of expression are not readily recognized using sectioned tissue owing to problems in visualization of signals with varying intensities. Herein, we describe the use of whole mount in situ hybridization for identification of cochlear longitudinal and radial expression gradients in the neurosensory epithelium, hair cells. Not only can these hair cell gradients be shown in adult tissues, but also the developmental up-regulation and down-regulation of genes and their associated spatio-temporal expression patterns can be demonstrated.

Original languageEnglish
Pages (from-to)65-76
Number of pages12
JournalBrain Research Protocols
Volume9
Issue number1
DOIs
StatePublished - 2002

Fingerprint

Cochlea
In Situ Hybridization
Organ of Corti
Inner Ear
Up-Regulation
Down-Regulation
Epithelium
Genes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Cochlear whole mount in situ hybridization : Identification of longitudinal and radial gradients. / Judice, Tiffany N.; Nelson, Nathan C.; Beisel, Chase L.; Delimont, Duane C.; Fritzsch, Bernd; Beisel, Kirk.

In: Brain Research Protocols, Vol. 9, No. 1, 2002, p. 65-76.

Research output: Contribution to journalArticle

Judice, Tiffany N. ; Nelson, Nathan C. ; Beisel, Chase L. ; Delimont, Duane C. ; Fritzsch, Bernd ; Beisel, Kirk. / Cochlear whole mount in situ hybridization : Identification of longitudinal and radial gradients. In: Brain Research Protocols. 2002 ; Vol. 9, No. 1. pp. 65-76.
@article{9979e9e8ec7d42e2a3cec5f7d5036804,
title = "Cochlear whole mount in situ hybridization: Identification of longitudinal and radial gradients",
abstract = "The morphology of the organ of Corti has a radial asymmetry and also changes longitudinally from base to apex. Cellular localization of transcripts within the inner ear has relied primarily on the use of sectioned tissue with in situ hybridization. However, radial and longitudinal gradients of expression are not readily recognized using sectioned tissue owing to problems in visualization of signals with varying intensities. Herein, we describe the use of whole mount in situ hybridization for identification of cochlear longitudinal and radial expression gradients in the neurosensory epithelium, hair cells. Not only can these hair cell gradients be shown in adult tissues, but also the developmental up-regulation and down-regulation of genes and their associated spatio-temporal expression patterns can be demonstrated.",
author = "Judice, {Tiffany N.} and Nelson, {Nathan C.} and Beisel, {Chase L.} and Delimont, {Duane C.} and Bernd Fritzsch and Kirk Beisel",
year = "2002",
doi = "10.1016/S1385-299X(01)00138-6",
language = "English",
volume = "9",
pages = "65--76",
journal = "Brain Research Protocols",
issn = "1385-299X",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Cochlear whole mount in situ hybridization

T2 - Identification of longitudinal and radial gradients

AU - Judice, Tiffany N.

AU - Nelson, Nathan C.

AU - Beisel, Chase L.

AU - Delimont, Duane C.

AU - Fritzsch, Bernd

AU - Beisel, Kirk

PY - 2002

Y1 - 2002

N2 - The morphology of the organ of Corti has a radial asymmetry and also changes longitudinally from base to apex. Cellular localization of transcripts within the inner ear has relied primarily on the use of sectioned tissue with in situ hybridization. However, radial and longitudinal gradients of expression are not readily recognized using sectioned tissue owing to problems in visualization of signals with varying intensities. Herein, we describe the use of whole mount in situ hybridization for identification of cochlear longitudinal and radial expression gradients in the neurosensory epithelium, hair cells. Not only can these hair cell gradients be shown in adult tissues, but also the developmental up-regulation and down-regulation of genes and their associated spatio-temporal expression patterns can be demonstrated.

AB - The morphology of the organ of Corti has a radial asymmetry and also changes longitudinally from base to apex. Cellular localization of transcripts within the inner ear has relied primarily on the use of sectioned tissue with in situ hybridization. However, radial and longitudinal gradients of expression are not readily recognized using sectioned tissue owing to problems in visualization of signals with varying intensities. Herein, we describe the use of whole mount in situ hybridization for identification of cochlear longitudinal and radial expression gradients in the neurosensory epithelium, hair cells. Not only can these hair cell gradients be shown in adult tissues, but also the developmental up-regulation and down-regulation of genes and their associated spatio-temporal expression patterns can be demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=0036178693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036178693&partnerID=8YFLogxK

U2 - 10.1016/S1385-299X(01)00138-6

DO - 10.1016/S1385-299X(01)00138-6

M3 - Article

VL - 9

SP - 65

EP - 76

JO - Brain Research Protocols

JF - Brain Research Protocols

SN - 1385-299X

IS - 1

ER -