TY - JOUR
T1 - Comparative evaluation of the Munt-Dash air-interface diffusion chamber and Franz chamber for the in vitro examination of topical spray formulations
AU - Munt, Daniel J.
AU - Qi, Yongyue
AU - Dash, Alekha K.
N1 - Funding Information:
Andrew Wilt and PermeGear Inc. (Hellertown, PA) provided design input and material construction of the Munt-Dash chamber. Construction of the syringe pump sprayer was done with the assistance of Brad Walters, Creighton University, Physics Department, Omaha, Nebraska. Powder x-ray diffraction studies were performed by Dr. Harsh Chauhan, Creighton University, Department of Pharmacy Sciences, Omaha, Nebraska. Powder x-ray diffraction experiments were performed in part in the Nebraska Nanoscale Facility: National Nanotechnology Coordinated Infrastructure and the Nebraska Center for Materials and Nanoscience, which are supported by the National Science Foundation under Award NNCI-1542182, and the Nebraska Research Initiative. University of Nebraska Lincoln, Lincoln, Nebraska.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/10/25
Y1 - 2021/10/25
N2 - In vitro diffusion testing of topical formulations has long been examined using Franz diffusion chambers, however, Franz chambers are typically used with relatively large volumes, lack the air/membrane interface present in vivo, and do not account for changes in formula characteristics as solvent evaporates. Here we present our patented Munt-Dash diffusion chamber designed for direct spray application onto a model membrane. Diffusion characteristics from topical spray formulations utilizing both the Munt-Dash chamber and Franz diffusion chambers were evaluated and compared. Using diclofenac sodium and lidocaine hydrochloride as model drugs and shed snakeskin as a model for stratum corneum, test solutions were applied to Franz diffusion chambers using a pipette and to the Munt-Dash chamber using a high-speed syringe pump and sprayer. Both chambers presented permeability data consistent with previously reported in vitro and in vivo studies. Significant differences were observed in permeability by formulation and temperature. This suggests that although Franz chambers produce relevant data, the failure to account for small volumes and drying during application may produce misleading results. The Munt-Dash chamber may improve in vitro testing by providing these factors. This data suggests the Munt-Dash chamber is a suitable alternative to the Franz chamber for topical spray formulations.
AB - In vitro diffusion testing of topical formulations has long been examined using Franz diffusion chambers, however, Franz chambers are typically used with relatively large volumes, lack the air/membrane interface present in vivo, and do not account for changes in formula characteristics as solvent evaporates. Here we present our patented Munt-Dash diffusion chamber designed for direct spray application onto a model membrane. Diffusion characteristics from topical spray formulations utilizing both the Munt-Dash chamber and Franz diffusion chambers were evaluated and compared. Using diclofenac sodium and lidocaine hydrochloride as model drugs and shed snakeskin as a model for stratum corneum, test solutions were applied to Franz diffusion chambers using a pipette and to the Munt-Dash chamber using a high-speed syringe pump and sprayer. Both chambers presented permeability data consistent with previously reported in vitro and in vivo studies. Significant differences were observed in permeability by formulation and temperature. This suggests that although Franz chambers produce relevant data, the failure to account for small volumes and drying during application may produce misleading results. The Munt-Dash chamber may improve in vitro testing by providing these factors. This data suggests the Munt-Dash chamber is a suitable alternative to the Franz chamber for topical spray formulations.
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U2 - 10.1016/j.ijpharm.2021.121127
DO - 10.1016/j.ijpharm.2021.121127
M3 - Article
C2 - 34560210
AN - SCOPUS:85115955008
VL - 608
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
M1 - 121127
ER -