Comparative genome hybridization reveals widespread aneuploidy in Candida albicans laboratory strains

Anna Selmecki, Sven Bergmann, Judith Berman

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Clinical strains of Candida albicans are highly tolerant of aneuploidies and other genome rearrangements. We have used comparative genome hybridization (CGH), in an array format, to analyse the copy number of over 6000 open reading frames (ORFs) in the genomic DNA of C. albicans laboratory strains carrying one (CAI-4) to three (BWP17) auxotrophies. We find that during disruption of the HIS1 locus all genes telomeric to HIS1 were deleted and telomeric repeats were added to a 9 nt sequence within the transforming DNA. This deletion occurred in ∼10% of transformants analysed and was stably maintained through two additional rounds of transformation and counterselection of the transformation marker. In one example, the deletion was repaired, apparently via break-induced replication. Furthermore, all CAI-4 strains tested were trisomie for chromosome 2 although this trisomy appears to be unstable, as it is not detected in strains subsequently derived from CAI-4. Our data indicate CGH arrays can be used to detect monosomies and trisomies, to predict the sites of chromosome breaks, and to identify chromosomal aberrations that have not been detected with other approaches in C. albicans strains. Furthermore, they highlight the high level of genome instability in C. albicans laboratory strains exposed to the stress of transformation and counterselection on 5-fluoro-orotic acid.

Original languageEnglish
Pages (from-to)1553-1565
Number of pages13
JournalMolecular Microbiology
Volume55
Issue number5
DOIs
StatePublished - Mar 2005

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carboxyamido-triazole
Comparative Genomic Hybridization
Aneuploidy
Candida albicans
Trisomy
Monosomy
Chromosome Breakage
Chromosomes, Human, Pair 2
Genomic Instability
DNA
Chromosome Aberrations
Open Reading Frames
Genome
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Microbiology

Cite this

Comparative genome hybridization reveals widespread aneuploidy in Candida albicans laboratory strains. / Selmecki, Anna; Bergmann, Sven; Berman, Judith.

In: Molecular Microbiology, Vol. 55, No. 5, 03.2005, p. 1553-1565.

Research output: Contribution to journalArticle

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