Comparison and contrast of affinity chromatographic determinations of plasma glycated albumin and total glycated plasma protein

Marc Rendell, Kathy Rasbold, Julia Nierenberg, Randy Krohn, Greg Hermanson, Dennis Klenk, Paul K. Smith

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Techniques for affinity measurement of glycated albumin and for glycated total plasma protein have been developed. The two techniques were contrasted. Both techniques are linear over a 100-fold range of sample concentrations. There appears to be a non-specific early glucose binding phase to non-albumin plasma proteins. Although this phase is detected by radioactive incorporation and thiobarbituric acid, it does not interfere with the affinity determination, which does not appear to detect the early binding species. The correlation of glycated albumin levels with glycated hemoglobin levels is much stronger than that of glycated globulin levels with glycated hemoglobin levels. Due to the large contribution of glycated albumin levels to total glycated serum protein levels, the correlation of the latter with glycated hemoglobin levels is sufficiently strong to allow the use of either technique as an adequate index of glycation.

Original languageEnglish
Pages (from-to)216-220
Number of pages5
JournalClinical Biochemistry
Volume19
Issue number4
DOIs
StatePublished - 1986

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Glycosylated Hemoglobin A
Serum Albumin
Blood Proteins
Plasmas
Globulins
Glucose
glycosylated serum albumin

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Comparison and contrast of affinity chromatographic determinations of plasma glycated albumin and total glycated plasma protein. / Rendell, Marc; Rasbold, Kathy; Nierenberg, Julia; Krohn, Randy; Hermanson, Greg; Klenk, Dennis; Smith, Paul K.

In: Clinical Biochemistry, Vol. 19, No. 4, 1986, p. 216-220.

Research output: Contribution to journalArticle

Rendell, Marc ; Rasbold, Kathy ; Nierenberg, Julia ; Krohn, Randy ; Hermanson, Greg ; Klenk, Dennis ; Smith, Paul K. / Comparison and contrast of affinity chromatographic determinations of plasma glycated albumin and total glycated plasma protein. In: Clinical Biochemistry. 1986 ; Vol. 19, No. 4. pp. 216-220.
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AU - Klenk, Dennis

AU - Smith, Paul K.

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