Complete genome sequence of the naegleria fowleri (strain lee) closed circular extrachromosomal ribosomal DNA element

John C. Mullican; Kristen M. Drescher; Nora M. Chapman; Steven Tracy

doi:10.1128/MRA.01055-20

Complete genome sequence of the naegleria fowleri (strain lee) closed circular extrachromosomal ribosomal DNA element

John C. Mullican, Kristen M. Drescher, Nora M. Chapman, Steven Tracy

Department of Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

The circular extrachromosomal ribosomal DNA (rDNA) element of Naegleria fowleri strain LEE was molecularly cloned and fully sequenced. The element comprises 15,786bp and contains a single copy of the organism's rDNA cistron. The nonribosomal sequence contains five potential open reading frames, two large direct repeat sequences, and numerous smaller repeated-sequence regions.

Original language	English (US)
Article number	e01055-20
Journal	Microbiology Resource Announcements
Volume	9
Issue number	49
DOIs	https://doi.org/10.1128/MRA.01055-20
State	Published - Dec 3 2020

All Science Journal Classification (ASJC) codes

Molecular Biology
Immunology and Microbiology (miscellaneous)
Genetics

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title = "Complete genome sequence of the naegleria fowleri (strain lee) closed circular extrachromosomal ribosomal DNA element",

abstract = "The circular extrachromosomal ribosomal DNA (rDNA) element of Naegleria fowleri strain LEE was molecularly cloned and fully sequenced. The element comprises 15,786bp and contains a single copy of the organism's rDNA cistron. The nonribosomal sequence contains five potential open reading frames, two large direct repeat sequences, and numerous smaller repeated-sequence regions.",

author = "Mullican, {John C.} and Drescher, {Kristen M.} and Chapman, {Nora M.} and Steven Tracy",

note = "Funding Information: This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sector. We thank Kevin Kendall (MacVector, Inc.) for assistance with the hybrid assembly of Sanger reads with NGS data.",

year = "2020",

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AU - Mullican, John C.

AU - Drescher, Kristen M.

AU - Chapman, Nora M.

AU - Tracy, Steven

N1 - Funding Information: This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sector. We thank Kevin Kendall (MacVector, Inc.) for assistance with the hybrid assembly of Sanger reads with NGS data.

PY - 2020/12/3

Y1 - 2020/12/3

N2 - The circular extrachromosomal ribosomal DNA (rDNA) element of Naegleria fowleri strain LEE was molecularly cloned and fully sequenced. The element comprises 15,786bp and contains a single copy of the organism's rDNA cistron. The nonribosomal sequence contains five potential open reading frames, two large direct repeat sequences, and numerous smaller repeated-sequence regions.

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