Abstract
Genetically engineered mice have greatly improved our understanding of gene functions and disease mechanisms. Nevertheless, the traditional knock-out approach has limitations in the overall viability of mutants. The application of the Cre/loxP system in the inner ear can help bypass this difficulty by generation of conditional gene recombineering. However, to do so requires an expression system that allows ear-specific temporally inducible, gene abrogation of one or more of the increasingly available floxed genes. To date, three approaches have been successfully used to create murine inner ear-specific Cre lines: conventional transgenesis, BAC transgenesis, and gene knock-in. Unfortunately, timing of conditional Cre activity does not extend beyond the regulatory range of the gene controlling Cre expression. Rectification of this problem requires the generation of tamoxifen or tetracycline inducible systems in the inner ear. Examination of integrase expression at different loci will facilitate studies on the expression of exogenous transgenes. These genetic applications for the mouse genome will dramatically advance in vivo gene function studies.
| Original language | English |
|---|---|
| Pages (from-to) | 243-254 |
| Number of pages | 12 |
| Journal | Brain Research |
| Volume | 1091 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 26 2006 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Clinical Neurology
- Developmental Biology
- Molecular Biology
Cite this
Conditional and inducible gene recombineering in the mouse inner ear. / Tian, Yong; James, Sally; Zuo, Jian; Fritzsch, Bernd; Beisel, Kirk.
In: Brain Research, Vol. 1091, No. 1, 26.05.2006, p. 243-254.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Conditional and inducible gene recombineering in the mouse inner ear
AU - Tian, Yong
AU - James, Sally
AU - Zuo, Jian
AU - Fritzsch, Bernd
AU - Beisel, Kirk
PY - 2006/5/26
Y1 - 2006/5/26
N2 - Genetically engineered mice have greatly improved our understanding of gene functions and disease mechanisms. Nevertheless, the traditional knock-out approach has limitations in the overall viability of mutants. The application of the Cre/loxP system in the inner ear can help bypass this difficulty by generation of conditional gene recombineering. However, to do so requires an expression system that allows ear-specific temporally inducible, gene abrogation of one or more of the increasingly available floxed genes. To date, three approaches have been successfully used to create murine inner ear-specific Cre lines: conventional transgenesis, BAC transgenesis, and gene knock-in. Unfortunately, timing of conditional Cre activity does not extend beyond the regulatory range of the gene controlling Cre expression. Rectification of this problem requires the generation of tamoxifen or tetracycline inducible systems in the inner ear. Examination of integrase expression at different loci will facilitate studies on the expression of exogenous transgenes. These genetic applications for the mouse genome will dramatically advance in vivo gene function studies.
AB - Genetically engineered mice have greatly improved our understanding of gene functions and disease mechanisms. Nevertheless, the traditional knock-out approach has limitations in the overall viability of mutants. The application of the Cre/loxP system in the inner ear can help bypass this difficulty by generation of conditional gene recombineering. However, to do so requires an expression system that allows ear-specific temporally inducible, gene abrogation of one or more of the increasingly available floxed genes. To date, three approaches have been successfully used to create murine inner ear-specific Cre lines: conventional transgenesis, BAC transgenesis, and gene knock-in. Unfortunately, timing of conditional Cre activity does not extend beyond the regulatory range of the gene controlling Cre expression. Rectification of this problem requires the generation of tamoxifen or tetracycline inducible systems in the inner ear. Examination of integrase expression at different loci will facilitate studies on the expression of exogenous transgenes. These genetic applications for the mouse genome will dramatically advance in vivo gene function studies.
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U2 - 10.1016/j.brainres.2006.01.040
DO - 10.1016/j.brainres.2006.01.040
M3 - Article
VL - 1091
SP - 243
EP - 254
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -