Conformation and Domain Movement Analysis of Human Matrix Metalloproteinase-2: Role of Associated Zn2+ and Ca2+ Ions

Leah Voit-Ostricki, Sándor Lovas, Charles R. Watts

Research output: Contribution to journalArticle

Abstract

Matrix metaloproteinase-2 (MMP-2) is an extracellular Zn2+ protease specific to type I and IV collagens. Its expression is associated with several inflammatory, degenerative, and malignant diseases. Conformational properties, domain movements, and interactions between MMP-2 and its associated metal ions were characterized using a 1.0 µs molecular dynamics simulation. Dihedral principle component analysis revealed ten families of conformations with the greatest degree of variability occurring in the link region connecting the catalytic and hemopexin domains. Dynamic cross-correlation analysis indicated domain movements corresponding to the opening and closing of the hemopexin domain in relation to the fibronectin and catalytic domains facilitated by the link region. Interaction energies were calculated using the molecular mechanics Poisson Boltzman surface area-interaction entropy (MMPBSA-IE) analysis method and revealed strong binding energies for the catalytic Zn2+ ion 1, Ca2+ ion 1, and Ca2+ ion 3 with significant conformational stability at the binding sites of Zn2+ ion 1 and Ca2+ ion 1. Ca2+ ion 2 diffuses freely away from its crystallographically defined binding site. Zn2+ ion 2 plays a minor role in conformational stability of the catalytic domain while Ca2+ ion 3 is strongly attracted to the highly electronegative sidechains of the Asp residues around the central β-sheet core of the hemopexin domain; however, the interacting residue sidechain carboxyl groups are outside of Ca2+ ion 3's coordination sphere.

Original languageEnglish (US)
JournalInternational journal of molecular sciences
Volume20
Issue number17
DOIs
StatePublished - Aug 27 2019
Externally publishedYes

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Conformations
Ions
matrices
Hemopexin
ions
Catalytic Domain
Binding sites
Binding Sites
human MMP2 protein
Metalloproteases
protease
Molecular mechanics
Collagen Type IV
carboxyl group
interactions
closing
collagens
Collagen Type I
Binding energy
Fibronectins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Conformation and Domain Movement Analysis of Human Matrix Metalloproteinase-2 : Role of Associated Zn2+ and Ca2+ Ions. / Voit-Ostricki, Leah; Lovas, Sándor; Watts, Charles R.

In: International journal of molecular sciences, Vol. 20, No. 17, 27.08.2019.

Research output: Contribution to journalArticle

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