Construction and Characterization of a NotI Linking Library from Human Chromosome Region 1q25-qter

Catherine B. Talmadge, Dong Kai Zhen, Ji Yi Wang, Peter Berglund, Bi Fang Li, Michael Weston, William J. Kimberling, Eugene R. Zabarovsky, Eric J. Stanbridge, George Klein, Janos Sumegi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chromosome 1q25-qter-specific NotI linking clones have been isolated from a NotI linking library that was constructed using DNA from MCH206.1 somatic cell hybrid cells. These cells contain chromosome 1q25-qter translocated to human chromosome Xp22 as the only human genetic material in mouse background. Sixty-eight NotI linking clones have been mapped by a combination of fluorescence in situ hybridization and R-banding to cytogenetic bands on the long arm of chromosome 1. The relative order of 11 Not I clones and their relation to known chromosome 1 markers have also been determined in 1q32 and 1q41, where the genes of Van der Woude and Usher syndrome type IIa have been previously mapped: cen-chr1.14-chr1.79-chr1.56-chr1.11-chr1.95-chr1.58 (chr1.74)-D1S70-chr1.15-chr1.82 (chr1,143) -chr1.62-D1S81 -tel. The 1q32- and 1q41-specific NotI linking clones were sequenced in the vicinity of the Not I site. They were analyzed in terms of nucleotide composition, G+C content, frequency of CpG dinucleotides, and protein coding potentials. Most of the 1q32-q41-specific NotI linking clones were derived from CpG islands. Sequences of three NotI linking clones proved to be identical with known genes. Six of the remaining eight had a high potential for coding regions and shared short homologous regions with sequences in the GenBank database. The Not I linking clones and the identified CpG islands will provide valuable resources for constructing a long-range restriction map of chromosome 1q25-q44 and for the eventual isolation of disease genes of Van der Woude syndrome (1q32-q41) and Usher syndrome type Ha (1q41).

Original languageEnglish
Pages (from-to)105-114
Number of pages10
JournalGenomics
Volume29
Issue number1
DOIs
StatePublished - Sep 1 1995

Fingerprint

Human Chromosomes
Libraries
Clone Cells
Usher Syndromes
CpG Islands
Hybrid Cells
Chromosomes
Chromosomes, Human, Pair 1
Genes
Nucleic Acid Databases
Medical Genetics
Base Composition
Fluorescence In Situ Hybridization
Genetic Markers
Cytogenetics
Nucleotides
Databases
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Talmadge, C. B., Zhen, D. K., Wang, J. Y., Berglund, P., Li, B. F., Weston, M., ... Sumegi, J. (1995). Construction and Characterization of a NotI Linking Library from Human Chromosome Region 1q25-qter. Genomics, 29(1), 105-114. https://doi.org/10.1006/geno.1995.1220

Construction and Characterization of a NotI Linking Library from Human Chromosome Region 1q25-qter. / Talmadge, Catherine B.; Zhen, Dong Kai; Wang, Ji Yi; Berglund, Peter; Li, Bi Fang; Weston, Michael; Kimberling, William J.; Zabarovsky, Eugene R.; Stanbridge, Eric J.; Klein, George; Sumegi, Janos.

In: Genomics, Vol. 29, No. 1, 01.09.1995, p. 105-114.

Research output: Contribution to journalArticle

Talmadge, CB, Zhen, DK, Wang, JY, Berglund, P, Li, BF, Weston, M, Kimberling, WJ, Zabarovsky, ER, Stanbridge, EJ, Klein, G & Sumegi, J 1995, 'Construction and Characterization of a NotI Linking Library from Human Chromosome Region 1q25-qter', Genomics, vol. 29, no. 1, pp. 105-114. https://doi.org/10.1006/geno.1995.1220
Talmadge, Catherine B. ; Zhen, Dong Kai ; Wang, Ji Yi ; Berglund, Peter ; Li, Bi Fang ; Weston, Michael ; Kimberling, William J. ; Zabarovsky, Eugene R. ; Stanbridge, Eric J. ; Klein, George ; Sumegi, Janos. / Construction and Characterization of a NotI Linking Library from Human Chromosome Region 1q25-qter. In: Genomics. 1995 ; Vol. 29, No. 1. pp. 105-114.
@article{d059dbe6a23749519f6e35eef8b50221,
title = "Construction and Characterization of a NotI Linking Library from Human Chromosome Region 1q25-qter",
abstract = "Chromosome 1q25-qter-specific NotI linking clones have been isolated from a NotI linking library that was constructed using DNA from MCH206.1 somatic cell hybrid cells. These cells contain chromosome 1q25-qter translocated to human chromosome Xp22 as the only human genetic material in mouse background. Sixty-eight NotI linking clones have been mapped by a combination of fluorescence in situ hybridization and R-banding to cytogenetic bands on the long arm of chromosome 1. The relative order of 11 Not I clones and their relation to known chromosome 1 markers have also been determined in 1q32 and 1q41, where the genes of Van der Woude and Usher syndrome type IIa have been previously mapped: cen-chr1.14-chr1.79-chr1.56-chr1.11-chr1.95-chr1.58 (chr1.74)-D1S70-chr1.15-chr1.82 (chr1,143) -chr1.62-D1S81 -tel. The 1q32- and 1q41-specific NotI linking clones were sequenced in the vicinity of the Not I site. They were analyzed in terms of nucleotide composition, G+C content, frequency of CpG dinucleotides, and protein coding potentials. Most of the 1q32-q41-specific NotI linking clones were derived from CpG islands. Sequences of three NotI linking clones proved to be identical with known genes. Six of the remaining eight had a high potential for coding regions and shared short homologous regions with sequences in the GenBank database. The Not I linking clones and the identified CpG islands will provide valuable resources for constructing a long-range restriction map of chromosome 1q25-q44 and for the eventual isolation of disease genes of Van der Woude syndrome (1q32-q41) and Usher syndrome type Ha (1q41).",
author = "Talmadge, {Catherine B.} and Zhen, {Dong Kai} and Wang, {Ji Yi} and Peter Berglund and Li, {Bi Fang} and Michael Weston and Kimberling, {William J.} and Zabarovsky, {Eugene R.} and Stanbridge, {Eric J.} and George Klein and Janos Sumegi",
year = "1995",
month = "9",
day = "1",
doi = "10.1006/geno.1995.1220",
language = "English",
volume = "29",
pages = "105--114",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Construction and Characterization of a NotI Linking Library from Human Chromosome Region 1q25-qter

AU - Talmadge, Catherine B.

AU - Zhen, Dong Kai

AU - Wang, Ji Yi

AU - Berglund, Peter

AU - Li, Bi Fang

AU - Weston, Michael

AU - Kimberling, William J.

AU - Zabarovsky, Eugene R.

AU - Stanbridge, Eric J.

AU - Klein, George

AU - Sumegi, Janos

PY - 1995/9/1

Y1 - 1995/9/1

N2 - Chromosome 1q25-qter-specific NotI linking clones have been isolated from a NotI linking library that was constructed using DNA from MCH206.1 somatic cell hybrid cells. These cells contain chromosome 1q25-qter translocated to human chromosome Xp22 as the only human genetic material in mouse background. Sixty-eight NotI linking clones have been mapped by a combination of fluorescence in situ hybridization and R-banding to cytogenetic bands on the long arm of chromosome 1. The relative order of 11 Not I clones and their relation to known chromosome 1 markers have also been determined in 1q32 and 1q41, where the genes of Van der Woude and Usher syndrome type IIa have been previously mapped: cen-chr1.14-chr1.79-chr1.56-chr1.11-chr1.95-chr1.58 (chr1.74)-D1S70-chr1.15-chr1.82 (chr1,143) -chr1.62-D1S81 -tel. The 1q32- and 1q41-specific NotI linking clones were sequenced in the vicinity of the Not I site. They were analyzed in terms of nucleotide composition, G+C content, frequency of CpG dinucleotides, and protein coding potentials. Most of the 1q32-q41-specific NotI linking clones were derived from CpG islands. Sequences of three NotI linking clones proved to be identical with known genes. Six of the remaining eight had a high potential for coding regions and shared short homologous regions with sequences in the GenBank database. The Not I linking clones and the identified CpG islands will provide valuable resources for constructing a long-range restriction map of chromosome 1q25-q44 and for the eventual isolation of disease genes of Van der Woude syndrome (1q32-q41) and Usher syndrome type Ha (1q41).

AB - Chromosome 1q25-qter-specific NotI linking clones have been isolated from a NotI linking library that was constructed using DNA from MCH206.1 somatic cell hybrid cells. These cells contain chromosome 1q25-qter translocated to human chromosome Xp22 as the only human genetic material in mouse background. Sixty-eight NotI linking clones have been mapped by a combination of fluorescence in situ hybridization and R-banding to cytogenetic bands on the long arm of chromosome 1. The relative order of 11 Not I clones and their relation to known chromosome 1 markers have also been determined in 1q32 and 1q41, where the genes of Van der Woude and Usher syndrome type IIa have been previously mapped: cen-chr1.14-chr1.79-chr1.56-chr1.11-chr1.95-chr1.58 (chr1.74)-D1S70-chr1.15-chr1.82 (chr1,143) -chr1.62-D1S81 -tel. The 1q32- and 1q41-specific NotI linking clones were sequenced in the vicinity of the Not I site. They were analyzed in terms of nucleotide composition, G+C content, frequency of CpG dinucleotides, and protein coding potentials. Most of the 1q32-q41-specific NotI linking clones were derived from CpG islands. Sequences of three NotI linking clones proved to be identical with known genes. Six of the remaining eight had a high potential for coding regions and shared short homologous regions with sequences in the GenBank database. The Not I linking clones and the identified CpG islands will provide valuable resources for constructing a long-range restriction map of chromosome 1q25-q44 and for the eventual isolation of disease genes of Van der Woude syndrome (1q32-q41) and Usher syndrome type Ha (1q41).

UR - http://www.scopus.com/inward/record.url?scp=0028982232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028982232&partnerID=8YFLogxK

U2 - 10.1006/geno.1995.1220

DO - 10.1006/geno.1995.1220

M3 - Article

VL - 29

SP - 105

EP - 114

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 1

ER -