Cryptosporidium parvum attachment to and internalization by human biliary epithelia in vitro: A morphologic study

Bing Q. Huang, Xian-Ming Chen, Nicholas F. LaRusso

Research output: Contribution to journalArticle

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Abstract

To explore the mechanisms by which Cryptosporidium parvum infects epithelial cells, we performed a detailed morphological study by serial electron microscopy to assess attachment to and internalization of biliary epithelial cells by C. parvum in an in vitro model of human biliary cryptosporidiosis. When C. parvum sporozoites initially attach to the host cell membrane, the rhoptry of the sporozoite extends to the attachment site; both micronemes and dense granules are recruited to the apical complex region of the attached parasite. During internalization, numerous vacuoles covered by the parasite's plasma membrane are formed and cluster together to establish a preparasitophorous vacuole. This preparasitophorous vacuole comes in contact with host cell membrane to form a host cell-parasite membrane interface, beneath which an electron-dense band begins to appear within the host cell cytoplasm. Simultaneously, host cells display membrane protrusion along the edge of the host cell-parasite membrane interface, resulting in the formation of a mature parasitophorous vacuole that completely covers the parasite. During internalization, vacuole-like structures appear in the apical complex region of the attached sporozoite, which bud out into host cells. A tunnel directly connecting the parasite to the host cell cytoplasm forms during internalization and remains when the parasite is totally internalized. Immunoelectron microscopy showed that sporozoite-associated proteins were localized along the dense band and at the parasitophorous vacuole membrane. These morphological observations provide evidence that secretion of parasite apical organelles and protrusion of host cell membrane play an important role in the attachment and internalization of host epithelial cells by C. parvum.

Original languageEnglish
Pages (from-to)212-221
Number of pages10
JournalJournal of Parasitology
Volume90
Issue number2
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Cryptosporidium parvum
parasite
Parasites
epithelium
Epithelium
Vacuoles
membrane
vacuoles
parasites
Sporozoites
sporozoites
Cell Membrane
Cell Surface Extensions
cell membranes
epithelial cells
Epithelial Cells
cells
cytoplasm
Cytoplasm
Cryptosporidiosis

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Cryptosporidium parvum attachment to and internalization by human biliary epithelia in vitro : A morphologic study. / Huang, Bing Q.; Chen, Xian-Ming; LaRusso, Nicholas F.

In: Journal of Parasitology, Vol. 90, No. 2, 04.2004, p. 212-221.

Research output: Contribution to journalArticle

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