Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513

Ai Yu Gong, Rui Zhou, Guoku Hu, Jun Liu, Danuta Sosnowska, Kristen M. Drescher, Haidong Dong, Xian-Ming Chen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translations suppression of B7-H1. Overexpression of miR-513 through transfection of miR513 precursor inhibited C. parvum-indaced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.

Original languageEnglish
Pages (from-to)160-169
Number of pages10
JournalJournal of Infectious Diseases
Volume201
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Cryptosporidium parvum
MicroRNAs
Parasites
Transfection
B7 Antigens
T-Lymphocytes
3' Untranslated Regions
Coculture Techniques
Infection
Neutralizing Antibodies
Cell Death
Epithelial Cells
Apoptosis
Proteins

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. / Gong, Ai Yu; Zhou, Rui; Hu, Guoku; Liu, Jun; Sosnowska, Danuta; Drescher, Kristen M.; Dong, Haidong; Chen, Xian-Ming.

In: Journal of Infectious Diseases, Vol. 201, No. 1, 01.2010, p. 160-169.

Research output: Contribution to journalArticle

Gong, Ai Yu ; Zhou, Rui ; Hu, Guoku ; Liu, Jun ; Sosnowska, Danuta ; Drescher, Kristen M. ; Dong, Haidong ; Chen, Xian-Ming. / Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. In: Journal of Infectious Diseases. 2010 ; Vol. 201, No. 1. pp. 160-169.
@article{7ece634b9716481e86afe286556b463c,
title = "Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513",
abstract = "Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translations suppression of B7-H1. Overexpression of miR-513 through transfection of miR513 precursor inhibited C. parvum-indaced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.",
author = "Gong, {Ai Yu} and Rui Zhou and Guoku Hu and Jun Liu and Danuta Sosnowska and Drescher, {Kristen M.} and Haidong Dong and Xian-Ming Chen",
year = "2010",
month = "1",
doi = "10.1086/648589",
language = "English",
volume = "201",
pages = "160--169",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513

AU - Gong, Ai Yu

AU - Zhou, Rui

AU - Hu, Guoku

AU - Liu, Jun

AU - Sosnowska, Danuta

AU - Drescher, Kristen M.

AU - Dong, Haidong

AU - Chen, Xian-Ming

PY - 2010/1

Y1 - 2010/1

N2 - Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translations suppression of B7-H1. Overexpression of miR-513 through transfection of miR513 precursor inhibited C. parvum-indaced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.

AB - Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translations suppression of B7-H1. Overexpression of miR-513 through transfection of miR513 precursor inhibited C. parvum-indaced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.

UR - http://www.scopus.com/inward/record.url?scp=71949093867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71949093867&partnerID=8YFLogxK

U2 - 10.1086/648589

DO - 10.1086/648589

M3 - Article

VL - 201

SP - 160

EP - 169

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 1

ER -