Cryptosporidium parvum is cytopathic for cultured human biliary epithelia via an apoptotic mechanism

Xian-Ming Chen, Susan A. Levine, Pamela Tietz, Eugene Krueger, Mark A. McNiven, Douglas M. Jefferson, Megan Mahle, Nicholas F. LaRusso

Research output: Contribution to journalArticle

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Abstract

While the clinical features of sclerosing cholangitis secondary to opportunistic infections of the biliary tree in patients with acquired immunodeficiency syndrome (AIDS) are well known, the mechanisms by which microbial pathogens such as Cryptosporidium parvum associated with this syndrome actually cause disease are obscure. We established an in vitro model of biliary cryptosporidiosis employing a human biliary epithelial cell line. Using morphological and biochemical techniques, we examined the interaction of C. parvum with cultured human cholangiocytes. When the apical plasma membrane of polarized, confluent monolayers of human biliary epithelial cells was exposed to C. parvum oocysts that had been excysted in vitro, sporozoites attached to and invaded the cells in a time-, dose-, temperature-, and pH- dependent manner. The infectious process was both plasma membrane domain- and cell-specific, because no attachment or invasion occurred when the basolateral membrane of cholangiocytes was exposed to the parasite, or when a human hepatocyte cell line (HepG2) was used. Time-lapse video microscopy and scanning electron microscopy (SEM) showed that sporozoite attachment was rapid, involved extensive cholangiocyte membrane ruffling, and culminated in parasite penetration into a tight-fitting vacuole formed by invagination of the plasma membrane similar to those found in naturally occurring infection in vivo. Transmission electron microscopy (TEM) showed that C. parvum organisms formed parasitophorus vacuoles and were able to undergo a complete reproductive cycle, forming both asexual and sexual reproductive stages. Unexpectedly, direct cytopathic effects were noted in infected monolayers, with widespread programmed cell death (i.e., apoptosis) of biliary epithelial cells as assessed both morphologically and biochemically beginning within hours after exposure to the organism. The novel finding of specific cytopathic invasion of biliary epithelia by C. parvum may be relevant to the pathogenesis and possible therapy of the secondary sclerosing cholangitis seen in AIDS patients with biliary cryptosporidiosis.

Original languageEnglish
Pages (from-to)906-913
Number of pages8
JournalHepatology
Volume28
Issue number4 I
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Cryptosporidium parvum
Epithelium
Cryptosporidiosis
Sporozoites
Sclerosing Cholangitis
Epithelial Cells
Cell Membrane
Vacuoles
Parasites
Acquired Immunodeficiency Syndrome
Cell Line
Video Microscopy
Membranes
Oocysts
Opportunistic Infections
Biliary Tract
Plasma Cells
Transmission Electron Microscopy
Electron Scanning Microscopy
Hepatocytes

All Science Journal Classification (ASJC) codes

  • Hepatology

Cite this

Chen, X-M., Levine, S. A., Tietz, P., Krueger, E., McNiven, M. A., Jefferson, D. M., ... LaRusso, N. F. (1998). Cryptosporidium parvum is cytopathic for cultured human biliary epithelia via an apoptotic mechanism. Hepatology, 28(4 I), 906-913. https://doi.org/10.1002/hep.510280402

Cryptosporidium parvum is cytopathic for cultured human biliary epithelia via an apoptotic mechanism. / Chen, Xian-Ming; Levine, Susan A.; Tietz, Pamela; Krueger, Eugene; McNiven, Mark A.; Jefferson, Douglas M.; Mahle, Megan; LaRusso, Nicholas F.

In: Hepatology, Vol. 28, No. 4 I, 1998, p. 906-913.

Research output: Contribution to journalArticle

Chen, X-M, Levine, SA, Tietz, P, Krueger, E, McNiven, MA, Jefferson, DM, Mahle, M & LaRusso, NF 1998, 'Cryptosporidium parvum is cytopathic for cultured human biliary epithelia via an apoptotic mechanism', Hepatology, vol. 28, no. 4 I, pp. 906-913. https://doi.org/10.1002/hep.510280402
Chen, Xian-Ming ; Levine, Susan A. ; Tietz, Pamela ; Krueger, Eugene ; McNiven, Mark A. ; Jefferson, Douglas M. ; Mahle, Megan ; LaRusso, Nicholas F. / Cryptosporidium parvum is cytopathic for cultured human biliary epithelia via an apoptotic mechanism. In: Hepatology. 1998 ; Vol. 28, No. 4 I. pp. 906-913.
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AB - While the clinical features of sclerosing cholangitis secondary to opportunistic infections of the biliary tree in patients with acquired immunodeficiency syndrome (AIDS) are well known, the mechanisms by which microbial pathogens such as Cryptosporidium parvum associated with this syndrome actually cause disease are obscure. We established an in vitro model of biliary cryptosporidiosis employing a human biliary epithelial cell line. Using morphological and biochemical techniques, we examined the interaction of C. parvum with cultured human cholangiocytes. When the apical plasma membrane of polarized, confluent monolayers of human biliary epithelial cells was exposed to C. parvum oocysts that had been excysted in vitro, sporozoites attached to and invaded the cells in a time-, dose-, temperature-, and pH- dependent manner. The infectious process was both plasma membrane domain- and cell-specific, because no attachment or invasion occurred when the basolateral membrane of cholangiocytes was exposed to the parasite, or when a human hepatocyte cell line (HepG2) was used. Time-lapse video microscopy and scanning electron microscopy (SEM) showed that sporozoite attachment was rapid, involved extensive cholangiocyte membrane ruffling, and culminated in parasite penetration into a tight-fitting vacuole formed by invagination of the plasma membrane similar to those found in naturally occurring infection in vivo. Transmission electron microscopy (TEM) showed that C. parvum organisms formed parasitophorus vacuoles and were able to undergo a complete reproductive cycle, forming both asexual and sexual reproductive stages. Unexpectedly, direct cytopathic effects were noted in infected monolayers, with widespread programmed cell death (i.e., apoptosis) of biliary epithelial cells as assessed both morphologically and biochemically beginning within hours after exposure to the organism. The novel finding of specific cytopathic invasion of biliary epithelia by C. parvum may be relevant to the pathogenesis and possible therapy of the secondary sclerosing cholangitis seen in AIDS patients with biliary cryptosporidiosis.

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