Decreased membrane fluidity and hyperpolarization in aluminum-treated PC-12 cells correlates with increased production of cellular oxidants

Victor J. Johnson, Masashi Tsunoda, Thomas F. Murray, Raghubir P. Sharma

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Effects of aluminum (Al) on membrane properties of excitable cells are not fully understood. Several reports have identified cellular membranes as sensitive targets for Al intoxication. In the present study, we tested the hypothesis that treatment with Al would alter membrane fluidity and potential and these changes would correlate with aberrant generation of cellular oxidants. The effects of in vitro Al exposure in resting rat pheochromocytoma (PC-12) cells, a model that exhibits neuron-like properties, were investigated. Treatment of PC-12 cells with Al (>0.01 mM) resulted in a concentration-dependent decrease in membrane fluidity. Similar concentrations of Al increased the rate of extracellular acidification, measured by a cytosensor microphysiometer, indicating stimulation of proton extrusion from cells. This change in proton extrusion was accompanied by a rapid and concentration- dependent hyperpolarizion of the cell membrane as determined by decreased fluorescence of a potential-sensitive dye, bis-[1,3-dibutylbarbituric acid]trimethine oxonol [Dibac 4(3)]. Al-induced perturbations of membrane properties correlated with an increased level of cellular oxidants, indicated by increasing dihydrorhodamine 123 oxidation. Results suggest that acute exposure to Al modifies membrane properties of neuron-like cells and therefore cellular membranes represent a plausible target for Al neurotoxicity. Alterations in membrane potential can have a dramatic impact on cellular communication especially in neurons and may be an important mechanism in Al neurotoxicity.

Original languageEnglish
Pages (from-to)221-230
Number of pages10
JournalEnvironmental Toxicology and Pharmacology
Volume19
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Fingerprint

Membrane Fluidity
Fluidity
Aluminum
Oxidants
Membranes
Neurons
Membrane Potentials
Extrusion
Protons
Cellular radio systems
Acidification
Pheochromocytoma
Cell membranes
Rats
Coloring Agents
Fluorescence
Communication
Cell Membrane
Oxidation

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Decreased membrane fluidity and hyperpolarization in aluminum-treated PC-12 cells correlates with increased production of cellular oxidants. / Johnson, Victor J.; Tsunoda, Masashi; Murray, Thomas F.; Sharma, Raghubir P.

In: Environmental Toxicology and Pharmacology, Vol. 19, No. 2, 02.2005, p. 221-230.

Research output: Contribution to journalArticle

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