Deletions in the fifth alpha helix of HIV-1 matrix block virus release

Bridget Sanford, Yan Li, Connor J. Maly, Christian J. Madson, Han Chen, You Zhou, Michael A. Belshan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The matrix (MA) protein of HIV-1 is the N-terminal component of the Gag structural protein and is critical for the early and late stages of viral replication. MA contains five α-helices (α1-α5). Deletions in the N-terminus of α5 as small as three amino acids impaired virus release. Electron microscopy of one deletion mutant (MAΔ96-120) showed that its particles were tethered to the surface of cells by membranous stalks. Immunoblots indicated all mutants were processed completely, but mutants with large deletions had alternative processing intermediates. Consistent with the EM data, MAΔ96-120 retained membrane association and multimerization capability. Co-expression of this mutant inhibited wild type particle release. Alanine scanning mutation in this region did not affect virus release, although the progeny virions were poorly infectious. Combined, these data demonstrate that structural ablation of the α5 of MA inhibits virus release.

Original languageEnglish
Pages (from-to)293-302
Number of pages10
JournalVirology
Volume468
DOIs
StatePublished - Nov 1 2014
Externally publishedYes

Fingerprint

Virus Release
HIV-1
gag Gene Products
Alanine
Virion
Electron Microscopy
Amino Acids
Mutation
Membranes
alpha-Helical Protein Conformation
Proteins

All Science Journal Classification (ASJC) codes

  • Virology
  • Medicine(all)

Cite this

Sanford, B., Li, Y., Maly, C. J., Madson, C. J., Chen, H., Zhou, Y., & Belshan, M. A. (2014). Deletions in the fifth alpha helix of HIV-1 matrix block virus release. Virology, 468, 293-302. https://doi.org/10.1016/j.virol.2014.08.017

Deletions in the fifth alpha helix of HIV-1 matrix block virus release. / Sanford, Bridget; Li, Yan; Maly, Connor J.; Madson, Christian J.; Chen, Han; Zhou, You; Belshan, Michael A.

In: Virology, Vol. 468, 01.11.2014, p. 293-302.

Research output: Contribution to journalArticle

Sanford, B, Li, Y, Maly, CJ, Madson, CJ, Chen, H, Zhou, Y & Belshan, MA 2014, 'Deletions in the fifth alpha helix of HIV-1 matrix block virus release', Virology, vol. 468, pp. 293-302. https://doi.org/10.1016/j.virol.2014.08.017
Sanford B, Li Y, Maly CJ, Madson CJ, Chen H, Zhou Y et al. Deletions in the fifth alpha helix of HIV-1 matrix block virus release. Virology. 2014 Nov 1;468:293-302. https://doi.org/10.1016/j.virol.2014.08.017
Sanford, Bridget ; Li, Yan ; Maly, Connor J. ; Madson, Christian J. ; Chen, Han ; Zhou, You ; Belshan, Michael A. / Deletions in the fifth alpha helix of HIV-1 matrix block virus release. In: Virology. 2014 ; Vol. 468. pp. 293-302.
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