Abstract
Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children. Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in C. parvum. Using an in vitro model of human intestinal cryptosporidiosis, we report here that some of these C. parvum RNA transcripts were selectively delivered into the nuclei of host epithelial cells during C. parvum infection. Nuclear delivery of several such parasitic RNAs, including Cdg7-FLc-0990, involved heat-shock protein 70mediated nuclear importing mechanism. Overexpression of Cdg7-FLc-0990 in intestinal epithelial cells resulted in significant changes in expression levels of specific genes, with significant overlapping with alterations in gene expression profile detected in host cells after C. parvum infection. Our data demonstrate that C. parvum transcripts of low protein-coding potential are selectively delivered into epithelial cells during infection and may modulate gene transcription in infected host cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 636-643 |
| Number of pages | 8 |
| Journal | Journal of Infectious Diseases |
| Volume | 215 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 15 2017 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Infectious Diseases
Cite this
Delivery of parasite RNA transcripts into infected epithelial cells during cryptosporidium infection and its potential impact on host gene transcription. / Wang, Yang; Gong, Ai Yu; Ma, Shibin; Chen, Xiqiang; Li, Yan; Su, Chun Jen; Norall, Dana; Chen, Jing; Strauss-Soukup, Juliane K.; Chen, Xian-Ming.
In: Journal of Infectious Diseases, Vol. 215, No. 4, 15.02.2017, p. 636-643.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Delivery of parasite RNA transcripts into infected epithelial cells during cryptosporidium infection and its potential impact on host gene transcription
AU - Wang, Yang
AU - Gong, Ai Yu
AU - Ma, Shibin
AU - Chen, Xiqiang
AU - Li, Yan
AU - Su, Chun Jen
AU - Norall, Dana
AU - Chen, Jing
AU - Strauss-Soukup, Juliane K.
AU - Chen, Xian-Ming
PY - 2017/2/15
Y1 - 2017/2/15
N2 - Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children. Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in C. parvum. Using an in vitro model of human intestinal cryptosporidiosis, we report here that some of these C. parvum RNA transcripts were selectively delivered into the nuclei of host epithelial cells during C. parvum infection. Nuclear delivery of several such parasitic RNAs, including Cdg7-FLc-0990, involved heat-shock protein 70mediated nuclear importing mechanism. Overexpression of Cdg7-FLc-0990 in intestinal epithelial cells resulted in significant changes in expression levels of specific genes, with significant overlapping with alterations in gene expression profile detected in host cells after C. parvum infection. Our data demonstrate that C. parvum transcripts of low protein-coding potential are selectively delivered into epithelial cells during infection and may modulate gene transcription in infected host cells.
AB - Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children. Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in C. parvum. Using an in vitro model of human intestinal cryptosporidiosis, we report here that some of these C. parvum RNA transcripts were selectively delivered into the nuclei of host epithelial cells during C. parvum infection. Nuclear delivery of several such parasitic RNAs, including Cdg7-FLc-0990, involved heat-shock protein 70mediated nuclear importing mechanism. Overexpression of Cdg7-FLc-0990 in intestinal epithelial cells resulted in significant changes in expression levels of specific genes, with significant overlapping with alterations in gene expression profile detected in host cells after C. parvum infection. Our data demonstrate that C. parvum transcripts of low protein-coding potential are selectively delivered into epithelial cells during infection and may modulate gene transcription in infected host cells.
UR - http://www.scopus.com/inward/record.url?scp=85017035838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017035838&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiw607
DO - 10.1093/infdis/jiw607
M3 - Article
C2 - 28007919
AN - SCOPUS:85017035838
VL - 215
SP - 636
EP - 643
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -