Delivery of parasite RNA transcripts into infected epithelial cells during cryptosporidium infection and its potential impact on host gene transcription

Yang Wang, Ai Yu Gong, Shibin Ma, Xiqiang Chen, Yan Li, Chun Jen Su, Dana Norall, Jing Chen, Juliane K. Strauss-Soukup, Xian-Ming Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children. Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in C. parvum. Using an in vitro model of human intestinal cryptosporidiosis, we report here that some of these C. parvum RNA transcripts were selectively delivered into the nuclei of host epithelial cells during C. parvum infection. Nuclear delivery of several such parasitic RNAs, including Cdg7-FLc-0990, involved heat-shock protein 70mediated nuclear importing mechanism. Overexpression of Cdg7-FLc-0990 in intestinal epithelial cells resulted in significant changes in expression levels of specific genes, with significant overlapping with alterations in gene expression profile detected in host cells after C. parvum infection. Our data demonstrate that C. parvum transcripts of low protein-coding potential are selectively delivered into epithelial cells during infection and may modulate gene transcription in infected host cells.

Original languageEnglish (US)
Pages (from-to)636-643
Number of pages8
JournalJournal of Infectious Diseases
Volume215
Issue number4
DOIs
StatePublished - Feb 15 2017

Fingerprint

Cryptosporidium parvum
Cryptosporidium
Parasites
Epithelial Cells
RNA
Infection
Genes
Cryptosporidiosis
Heat-Shock Proteins
Transcriptome
Diarrhea
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Delivery of parasite RNA transcripts into infected epithelial cells during cryptosporidium infection and its potential impact on host gene transcription. / Wang, Yang; Gong, Ai Yu; Ma, Shibin; Chen, Xiqiang; Li, Yan; Su, Chun Jen; Norall, Dana; Chen, Jing; Strauss-Soukup, Juliane K.; Chen, Xian-Ming.

In: Journal of Infectious Diseases, Vol. 215, No. 4, 15.02.2017, p. 636-643.

Research output: Contribution to journalArticle

Wang, Yang ; Gong, Ai Yu ; Ma, Shibin ; Chen, Xiqiang ; Li, Yan ; Su, Chun Jen ; Norall, Dana ; Chen, Jing ; Strauss-Soukup, Juliane K. ; Chen, Xian-Ming. / Delivery of parasite RNA transcripts into infected epithelial cells during cryptosporidium infection and its potential impact on host gene transcription. In: Journal of Infectious Diseases. 2017 ; Vol. 215, No. 4. pp. 636-643.
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