Abstract
The effect of jet propellant-8 (JP-8) fuel exposure on the biophysical, macroscopic and microscopic changes in vitro in porcine skin has been investigated. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the biophysical changes in stratum corneum (SC) lipid and protein. FTIR results showed that the treatment of the SC with JP-8 to increasing exposure time caused correspondingly greater percent decrease in the peak heights and areas under the absorbance curve of methylene and amide absorbances, suggesting greater loss of lipid and protein from SC layers. In vitro transepidermal water loss (TEWL) studies allowed an investigation into the macroscopic barrier properties of the skin. TEWL results were in consonance with that of FTIR. There was a significant increase (P <0.05) in TEWL through 8 and 24 h JP-8 exposed skin in comparison to the control. Light microscopy provided direct, corroborative, visual evidences of epidermal and dermal alterations. Epidermal swelling, dermal matrix granulation, mast cell granules, shortened collagen fibers were observed in the skin exposed with JP-8. Thus, it is concluded that JP-8 exposure causes appreciable biophysical and histological changes along with increased TEWL values in vitro in pig skin which may lead to skin irritation and dermal toxicity in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 123-131 |
| Number of pages | 9 |
| Journal | Environmental Toxicology and Pharmacology |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2001 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Toxicology
- Health, Toxicology and Mutagenesis
- Pharmacology
Cite this
Dermal toxicity : Effect of jet propellant-8 fuel exposure on the biophysical, macroscopic and microscopic properties of porcine skin. / Singh, Somnath; Singh, Jagdish.
In: Environmental Toxicology and Pharmacology, Vol. 10, No. 3, 2001, p. 123-131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dermal toxicity
T2 - Effect of jet propellant-8 fuel exposure on the biophysical, macroscopic and microscopic properties of porcine skin
AU - Singh, Somnath
AU - Singh, Jagdish
PY - 2001
Y1 - 2001
N2 - The effect of jet propellant-8 (JP-8) fuel exposure on the biophysical, macroscopic and microscopic changes in vitro in porcine skin has been investigated. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the biophysical changes in stratum corneum (SC) lipid and protein. FTIR results showed that the treatment of the SC with JP-8 to increasing exposure time caused correspondingly greater percent decrease in the peak heights and areas under the absorbance curve of methylene and amide absorbances, suggesting greater loss of lipid and protein from SC layers. In vitro transepidermal water loss (TEWL) studies allowed an investigation into the macroscopic barrier properties of the skin. TEWL results were in consonance with that of FTIR. There was a significant increase (P <0.05) in TEWL through 8 and 24 h JP-8 exposed skin in comparison to the control. Light microscopy provided direct, corroborative, visual evidences of epidermal and dermal alterations. Epidermal swelling, dermal matrix granulation, mast cell granules, shortened collagen fibers were observed in the skin exposed with JP-8. Thus, it is concluded that JP-8 exposure causes appreciable biophysical and histological changes along with increased TEWL values in vitro in pig skin which may lead to skin irritation and dermal toxicity in vivo.
AB - The effect of jet propellant-8 (JP-8) fuel exposure on the biophysical, macroscopic and microscopic changes in vitro in porcine skin has been investigated. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the biophysical changes in stratum corneum (SC) lipid and protein. FTIR results showed that the treatment of the SC with JP-8 to increasing exposure time caused correspondingly greater percent decrease in the peak heights and areas under the absorbance curve of methylene and amide absorbances, suggesting greater loss of lipid and protein from SC layers. In vitro transepidermal water loss (TEWL) studies allowed an investigation into the macroscopic barrier properties of the skin. TEWL results were in consonance with that of FTIR. There was a significant increase (P <0.05) in TEWL through 8 and 24 h JP-8 exposed skin in comparison to the control. Light microscopy provided direct, corroborative, visual evidences of epidermal and dermal alterations. Epidermal swelling, dermal matrix granulation, mast cell granules, shortened collagen fibers were observed in the skin exposed with JP-8. Thus, it is concluded that JP-8 exposure causes appreciable biophysical and histological changes along with increased TEWL values in vitro in pig skin which may lead to skin irritation and dermal toxicity in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0034903388&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034903388&partnerID=8YFLogxK
U2 - 10.1016/S1382-6689(01)00092-8
DO - 10.1016/S1382-6689(01)00092-8
M3 - Article
VL - 10
SP - 123
EP - 131
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
SN - 1382-6689
IS - 3
ER -