Abstract
Trace aluminum determination in biological material is difficult because of the very low levels generally present and the fact that significant quantities of aluminum are present in all reagents and the laboratory environment. A cation exchange chromatography procedure is outlined for the simple and quantitative determination of trace amounts of aluminum (i.e., ≥ 0.05 μg Al/ml urine) in biological material, employing 28Al neutron activation analysis. The procedure, utilizing a low power nuclear reactor ( 3.1 x 10 11 n/cm 2 s), consists of a) wet digestion of the biological material, b) cation exchange chromatography employing 1 M nitric acid to remove the major radiocontaminants sodium, chloride, silicate, and phosphate ions, and c) irradiation and radioassay of the aluminum contained in the resin. The aluminum concentration in normal urine samples varied from <0.05 to 0.13 μg/Al/ml. the precision of this method is such that we can measure with confidence aluminum levels of 0.05 μg/ml or above.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1084-1088 |
| Number of pages | 5 |
| Journal | Analytical Chemistry |
| Volume | 48 |
| Issue number | 7 |
| DOIs | |
| State | Published - 1976 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Determination of trace aluminum in urine by neutron activation analysis. / Blotcky, A. J.; Hobson, D.; Leffler, J. A.; Rack, E. P.; Recker, Robert R.
In: Analytical Chemistry, Vol. 48, No. 7, 1976, p. 1084-1088.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of trace aluminum in urine by neutron activation analysis
AU - Blotcky, A. J.
AU - Hobson, D.
AU - Leffler, J. A.
AU - Rack, E. P.
AU - Recker, Robert R.
PY - 1976
Y1 - 1976
N2 - Trace aluminum determination in biological material is difficult because of the very low levels generally present and the fact that significant quantities of aluminum are present in all reagents and the laboratory environment. A cation exchange chromatography procedure is outlined for the simple and quantitative determination of trace amounts of aluminum (i.e., ≥ 0.05 μg Al/ml urine) in biological material, employing 28Al neutron activation analysis. The procedure, utilizing a low power nuclear reactor ( 3.1 x 10 11 n/cm 2 s), consists of a) wet digestion of the biological material, b) cation exchange chromatography employing 1 M nitric acid to remove the major radiocontaminants sodium, chloride, silicate, and phosphate ions, and c) irradiation and radioassay of the aluminum contained in the resin. The aluminum concentration in normal urine samples varied from <0.05 to 0.13 μg/Al/ml. the precision of this method is such that we can measure with confidence aluminum levels of 0.05 μg/ml or above.
AB - Trace aluminum determination in biological material is difficult because of the very low levels generally present and the fact that significant quantities of aluminum are present in all reagents and the laboratory environment. A cation exchange chromatography procedure is outlined for the simple and quantitative determination of trace amounts of aluminum (i.e., ≥ 0.05 μg Al/ml urine) in biological material, employing 28Al neutron activation analysis. The procedure, utilizing a low power nuclear reactor ( 3.1 x 10 11 n/cm 2 s), consists of a) wet digestion of the biological material, b) cation exchange chromatography employing 1 M nitric acid to remove the major radiocontaminants sodium, chloride, silicate, and phosphate ions, and c) irradiation and radioassay of the aluminum contained in the resin. The aluminum concentration in normal urine samples varied from <0.05 to 0.13 μg/Al/ml. the precision of this method is such that we can measure with confidence aluminum levels of 0.05 μg/ml or above.
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U2 - 10.1021/ac60371a028
DO - 10.1021/ac60371a028
M3 - Article
VL - 48
SP - 1084
EP - 1088
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 7
ER -