Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer; Mark D. Reisbig; Nancy D. Hanson

doi:10.1128/JCM.02038-12

Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer, Mark D. Reisbig, Nancy D. Hanson

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

Original language	English (US)
Pages (from-to)	3722-3725
Number of pages	4
Journal	Journal of clinical microbiology
Volume	50
Issue number	11
DOIs	https://doi.org/10.1128/JCM.02038-12
State	Published - Nov 2012

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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title = "Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes",

abstract = "A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.",

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