Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer, Mark D. Reisbig, Nancy D. Hanson

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

Original languageEnglish (US)
Pages (from-to)3722-3725
Number of pages4
JournalJournal of clinical microbiology
Volume50
Issue number11
DOIs
StatePublished - Nov 1 2012

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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