Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer; Mark Reisbig; Nancy D. Hanson

doi:10.1128/JCM.02038-12

Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer, Mark Reisbig, Nancy D. Hanson

Research output: Contribution to journal › Article

13 Scopus citations

Abstract

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

Original language	English
Pages (from-to)	3722-3725
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	11
DOIs	https://doi.org/10.1128/JCM.02038-12
Publication status	Published - Nov 2012

Fingerprint

All Science Journal Classification (ASJC) codes

Microbiology (medical)

Cite this

Geyer, C. N., Reisbig, M., & Hanson, N. D. (2012). Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes. Journal of Clinical Microbiology, 50(11), 3722-3725. https://doi.org/10.1128/JCM.02038-12

@article{c02951fc01fb4b418e8ee9d81386a9fa,

title = "Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes",

abstract = "A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100{\%} when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.",

author = "Geyer, {Chelsie N.} and Mark Reisbig and Hanson, {Nancy D.}",

year = "2012",

month = "11",

doi = "10.1128/JCM.02038-12",

language = "English",

volume = "50",

pages = "3722--3725",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

AU - Geyer, Chelsie N.

AU - Reisbig, Mark

AU - Hanson, Nancy D.

PY - 2012/11

Y1 - 2012/11

N2 - A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

AB - A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

UR - http://www.scopus.com/inward/record.url?scp=84867521186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867521186&partnerID=8YFLogxK

U2 - 10.1128/JCM.02038-12

DO - 10.1128/JCM.02038-12

M3 - Article

C2 - 22895035

AN - SCOPUS:84867521186

VL - 50

SP - 3722

EP - 3725

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 11

ER -

Access to Document

10.1128/JCM.02038-12