Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer, Mark Reisbig, Nancy D. Hanson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

Original languageEnglish
Pages (from-to)3722-3725
Number of pages4
JournalJournal of Clinical Microbiology
Volume50
Issue number11
DOIs
StatePublished - Nov 2012

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Multiplex Polymerase Chain Reaction
Microbiology
Plasmids
Sensitivity and Specificity
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes. / Geyer, Chelsie N.; Reisbig, Mark; Hanson, Nancy D.

In: Journal of Clinical Microbiology, Vol. 50, No. 11, 11.2012, p. 3722-3725.

Research output: Contribution to journalArticle

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