Development of an in vitro dissolution method using microdialysis sampling technique for implantable drug delivery systems

Alekha K. Dash, Paul W. Haney, Marc J. Garavalia

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The major challenge faced during the development of implantable dosage forms for site-specific delivery is monitoring the local concentration of the drug at or around the site of action. The tissue concentration at the site is generally measured by either sacrificing the animal at different points in time or by determining the amount of drug left in the implants at various time intervals. Unfortunately, there are no official in vitro dissolution methods available to study the release characteristics of drugs from this drug delivery system. The objective of this investigation was to develop a simple method using microdialysis sampling technique to serve as an in vitro dissolution method for implantable drug delivery systems. Ciprofloxacin implants were prepared by compressing ciprofloxacin microcapsules in poly(lactic acid) (PLA) and poly(lactic-glycolic acid) (PLGA). A sensitive HPLC method was developed and validated for the assay of Ciprofloxacin. An in vitro dissolution method was developed to study the release characteristics of drug from these implants. The method used a microdialysis sampling technique and a small sample volume of release medium. The various advantages and disadvantages of this method over other USP methods are discussed.

Original languageEnglish
Pages (from-to)1036-1040
Number of pages5
JournalJournal of Pharmaceutical Sciences
Volume88
Issue number10
DOIs
StatePublished - 1999

Fingerprint

Implantable Infusion Pumps
Microdialysis
Ciprofloxacin
Dissolution
glycolic acid
Sampling
Drug Implants
Pharmaceutical Preparations
Dosage Forms
Lactic acid
Capsules
Assays
Animals
Tissue
Monitoring
Drug Delivery Systems
In Vitro Techniques
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science

Cite this

Development of an in vitro dissolution method using microdialysis sampling technique for implantable drug delivery systems. / Dash, Alekha K.; Haney, Paul W.; Garavalia, Marc J.

In: Journal of Pharmaceutical Sciences, Vol. 88, No. 10, 1999, p. 1036-1040.

Research output: Contribution to journalArticle

@article{a113326d66844d23a6a6be22a8ce86f4,
title = "Development of an in vitro dissolution method using microdialysis sampling technique for implantable drug delivery systems",
abstract = "The major challenge faced during the development of implantable dosage forms for site-specific delivery is monitoring the local concentration of the drug at or around the site of action. The tissue concentration at the site is generally measured by either sacrificing the animal at different points in time or by determining the amount of drug left in the implants at various time intervals. Unfortunately, there are no official in vitro dissolution methods available to study the release characteristics of drugs from this drug delivery system. The objective of this investigation was to develop a simple method using microdialysis sampling technique to serve as an in vitro dissolution method for implantable drug delivery systems. Ciprofloxacin implants were prepared by compressing ciprofloxacin microcapsules in poly(lactic acid) (PLA) and poly(lactic-glycolic acid) (PLGA). A sensitive HPLC method was developed and validated for the assay of Ciprofloxacin. An in vitro dissolution method was developed to study the release characteristics of drug from these implants. The method used a microdialysis sampling technique and a small sample volume of release medium. The various advantages and disadvantages of this method over other USP methods are discussed.",
author = "Dash, {Alekha K.} and Haney, {Paul W.} and Garavalia, {Marc J.}",
year = "1999",
doi = "10.1021/js980480g",
language = "English",
volume = "88",
pages = "1036--1040",
journal = "Journal of Pharmaceutical Sciences",
issn = "0022-3549",
publisher = "John Wiley and Sons Inc.",
number = "10",

}

TY - JOUR

T1 - Development of an in vitro dissolution method using microdialysis sampling technique for implantable drug delivery systems

AU - Dash, Alekha K.

AU - Haney, Paul W.

AU - Garavalia, Marc J.

PY - 1999

Y1 - 1999

N2 - The major challenge faced during the development of implantable dosage forms for site-specific delivery is monitoring the local concentration of the drug at or around the site of action. The tissue concentration at the site is generally measured by either sacrificing the animal at different points in time or by determining the amount of drug left in the implants at various time intervals. Unfortunately, there are no official in vitro dissolution methods available to study the release characteristics of drugs from this drug delivery system. The objective of this investigation was to develop a simple method using microdialysis sampling technique to serve as an in vitro dissolution method for implantable drug delivery systems. Ciprofloxacin implants were prepared by compressing ciprofloxacin microcapsules in poly(lactic acid) (PLA) and poly(lactic-glycolic acid) (PLGA). A sensitive HPLC method was developed and validated for the assay of Ciprofloxacin. An in vitro dissolution method was developed to study the release characteristics of drug from these implants. The method used a microdialysis sampling technique and a small sample volume of release medium. The various advantages and disadvantages of this method over other USP methods are discussed.

AB - The major challenge faced during the development of implantable dosage forms for site-specific delivery is monitoring the local concentration of the drug at or around the site of action. The tissue concentration at the site is generally measured by either sacrificing the animal at different points in time or by determining the amount of drug left in the implants at various time intervals. Unfortunately, there are no official in vitro dissolution methods available to study the release characteristics of drugs from this drug delivery system. The objective of this investigation was to develop a simple method using microdialysis sampling technique to serve as an in vitro dissolution method for implantable drug delivery systems. Ciprofloxacin implants were prepared by compressing ciprofloxacin microcapsules in poly(lactic acid) (PLA) and poly(lactic-glycolic acid) (PLGA). A sensitive HPLC method was developed and validated for the assay of Ciprofloxacin. An in vitro dissolution method was developed to study the release characteristics of drug from these implants. The method used a microdialysis sampling technique and a small sample volume of release medium. The various advantages and disadvantages of this method over other USP methods are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0032862121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032862121&partnerID=8YFLogxK

U2 - 10.1021/js980480g

DO - 10.1021/js980480g

M3 - Article

VL - 88

SP - 1036

EP - 1040

JO - Journal of Pharmaceutical Sciences

JF - Journal of Pharmaceutical Sciences

SN - 0022-3549

IS - 10

ER -