Different signal responses to lamprey GnRH-III in human cancer cells

Krisztina Herédi-Szabó, Richard F. Murphy, Sándor Lovas

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Analogs of GnRH used for the treatment of sex steroid-independent tumors are not optimal because of endocrine side-effects. Lamprey gonadotropin- releasing hormone III (lGnRH-III), however, directly inhibits the growth of several tumor cell lines derived from reproductive organs. In this study, the signaling and antiproliferative effects of lGnRH-III on two breast, a colonic and a pancreatic cancer cell lines were investigated. Binding affinity of the peptide was determined by competitive receptor binding assays. Cell proliferation was measured following treatment for 3 days with lGnRH-III. Intracellular cAMP levels in response to lGnRH-III binding were quantified and effects of pertussis toxin (PTX), an inhibitor of Gi activation, on suppression of growth by lGnRH-III were examined. lGnRH-III had comparable affinities for receptors on all four cell lines and inhibited their growth at micromolar concentrations in a dose-dependent manner. PTX-sensitive decrease in cAMP levels in response to lGnRH-III in colonic and pancreatic cells was observed, while lGnRH-III increased intracellular [Ca2+] in both breast cancer cell lines. This study further indicates that the same receptors utilize different signal transduction pathways in different cancer cells.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalInternational Journal of Peptide Research and Therapeutics
Volume12
Issue number4
DOIs
StatePublished - Dec 2006

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Lampreys
Cells
Neoplasms
Pertussis Toxin
Cell Line
Tumors
Growth
Signal transduction
Competitive Binding
gonadotropin-releasing hormone-III
Gonadotropin-Releasing Hormone
Cell proliferation
Tumor Cell Line
Pancreatic Neoplasms
Assays
Signal Transduction
Peptides
Breast
Chemical activation
Steroids

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Different signal responses to lamprey GnRH-III in human cancer cells. / Herédi-Szabó, Krisztina; Murphy, Richard F.; Lovas, Sándor.

In: International Journal of Peptide Research and Therapeutics, Vol. 12, No. 4, 12.2006, p. 359-364.

Research output: Contribution to journalArticle

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