Abstract
Compartmentalization of β-tubulin isotypes within cells according to function was examined in gerbil olfactory and respiratory epithelia by using specific antibodies to four β-tubulin isotypes (βI, βII, βIII, and βIV). Isotype synthesis was cell-type-specific, but the localization of the isotypes was not compartmentalized. All four isotypes were found in the cilia, dendrites, somata, and axons of olfactory neurons. Only two isotypes (βI and βIV) were present in the cilia of nasal respiratory epithelial cells. The βIV isotype, thought to be an essential component of cilia, was present in olfactory neurons and respiratory epithelial cells, which are ciliated, but was not found in basal cells (the stem cells of olfactory sensory neurons, which have no cilia). Olfactory neurons therefore do not synthesize βIV-tubulin until they mature, when functioning cilia are also elaborated. The failure to observe compartmentalization of β-tubulin isotypes in olfactory neurons sheds new light on potential functions of the β-tubulin isotypes.
| Original language | English |
|---|---|
| Pages (from-to) | 331-335 |
| Number of pages | 5 |
| Journal | Cell and Tissue Research |
| Volume | 309 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
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All Science Journal Classification (ASJC) codes
- Anatomy
- Clinical Biochemistry
- Cell Biology
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Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia. / Woo, Karen; Jensen-Smith, Heather C.; Ludueña, Richard F.; Hallworth, Richard J.
In: Cell and Tissue Research, Vol. 309, No. 2, 2002, p. 331-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia
AU - Woo, Karen
AU - Jensen-Smith, Heather C.
AU - Ludueña, Richard F.
AU - Hallworth, Richard J.
PY - 2002
Y1 - 2002
N2 - Compartmentalization of β-tubulin isotypes within cells according to function was examined in gerbil olfactory and respiratory epithelia by using specific antibodies to four β-tubulin isotypes (βI, βII, βIII, and βIV). Isotype synthesis was cell-type-specific, but the localization of the isotypes was not compartmentalized. All four isotypes were found in the cilia, dendrites, somata, and axons of olfactory neurons. Only two isotypes (βI and βIV) were present in the cilia of nasal respiratory epithelial cells. The βIV isotype, thought to be an essential component of cilia, was present in olfactory neurons and respiratory epithelial cells, which are ciliated, but was not found in basal cells (the stem cells of olfactory sensory neurons, which have no cilia). Olfactory neurons therefore do not synthesize βIV-tubulin until they mature, when functioning cilia are also elaborated. The failure to observe compartmentalization of β-tubulin isotypes in olfactory neurons sheds new light on potential functions of the β-tubulin isotypes.
AB - Compartmentalization of β-tubulin isotypes within cells according to function was examined in gerbil olfactory and respiratory epithelia by using specific antibodies to four β-tubulin isotypes (βI, βII, βIII, and βIV). Isotype synthesis was cell-type-specific, but the localization of the isotypes was not compartmentalized. All four isotypes were found in the cilia, dendrites, somata, and axons of olfactory neurons. Only two isotypes (βI and βIV) were present in the cilia of nasal respiratory epithelial cells. The βIV isotype, thought to be an essential component of cilia, was present in olfactory neurons and respiratory epithelial cells, which are ciliated, but was not found in basal cells (the stem cells of olfactory sensory neurons, which have no cilia). Olfactory neurons therefore do not synthesize βIV-tubulin until they mature, when functioning cilia are also elaborated. The failure to observe compartmentalization of β-tubulin isotypes in olfactory neurons sheds new light on potential functions of the β-tubulin isotypes.
UR - http://www.scopus.com/inward/record.url?scp=0036024993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036024993&partnerID=8YFLogxK
U2 - 10.1007/s00441-002-0591-2
DO - 10.1007/s00441-002-0591-2
M3 - Article
C2 - 12172793
AN - SCOPUS:0036024993
VL - 309
SP - 331
EP - 335
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 2
ER -