Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia

Karen Woo, Heather C. Jensen-Smith, Richard F. Ludueña, Richard J. Hallworth

Research output: Contribution to journalArticle

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Abstract

Compartmentalization of β-tubulin isotypes within cells according to function was examined in gerbil olfactory and respiratory epithelia by using specific antibodies to four β-tubulin isotypes (βI, βII, βIII, and βIV). Isotype synthesis was cell-type-specific, but the localization of the isotypes was not compartmentalized. All four isotypes were found in the cilia, dendrites, somata, and axons of olfactory neurons. Only two isotypes (βI and βIV) were present in the cilia of nasal respiratory epithelial cells. The βIV isotype, thought to be an essential component of cilia, was present in olfactory neurons and respiratory epithelial cells, which are ciliated, but was not found in basal cells (the stem cells of olfactory sensory neurons, which have no cilia). Olfactory neurons therefore do not synthesize βIV-tubulin until they mature, when functioning cilia are also elaborated. The failure to observe compartmentalization of β-tubulin isotypes in olfactory neurons sheds new light on potential functions of the β-tubulin isotypes.

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalCell and Tissue Research
Volume309
Issue number2
DOIs
StatePublished - 2002

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Gerbillinae
Nasal Mucosa
Cilia
Tubulin
Neurons
Epithelial Cells
Olfactory Receptor Neurons
Olfactory Mucosa
Respiratory Mucosa
Carisoprodol
Dendrites
Stem cells
Nose
Axons
Stem Cells
Cells
Antibodies

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Woo, K., Jensen-Smith, H. C., Ludueña, R. F., & Hallworth, R. J. (2002). Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia. Cell and Tissue Research, 309(2), 331-335. https://doi.org/10.1007/s00441-002-0591-2

Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia. / Woo, Karen; Jensen-Smith, Heather C.; Ludueña, Richard F.; Hallworth, Richard J.

In: Cell and Tissue Research, Vol. 309, No. 2, 2002, p. 331-335.

Research output: Contribution to journalArticle

Woo, K, Jensen-Smith, HC, Ludueña, RF & Hallworth, RJ 2002, 'Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia', Cell and Tissue Research, vol. 309, no. 2, pp. 331-335. https://doi.org/10.1007/s00441-002-0591-2
Woo, Karen ; Jensen-Smith, Heather C. ; Ludueña, Richard F. ; Hallworth, Richard J. / Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia. In: Cell and Tissue Research. 2002 ; Vol. 309, No. 2. pp. 331-335.
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