Diffraction imaging of spheres and melanoma cells with a microscope objective

Kenneth M. Jacobs, Li V. Yang, Junhua Ding, Andrew E. Ekpenyong, Reid Castellone, Jun Q. Lu, Xin Hua Hu

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.

Original languageEnglish (US)
Pages (from-to)521-527
Number of pages7
JournalJournal of Biophotonics
Volume2
Issue number8-9
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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  • Cite this

    Jacobs, K. M., Yang, L. V., Ding, J., Ekpenyong, A. E., Castellone, R., Lu, J. Q., & Hu, X. H. (2009). Diffraction imaging of spheres and melanoma cells with a microscope objective. Journal of Biophotonics, 2(8-9), 521-527. https://doi.org/10.1002/jbio.200910044