Diffraction imaging of spheres and melanoma cells with a microscope objective

Kenneth M. Jacobs, Li V. Yang, Junhua Ding, Andrew Ekpenyong, Reid Castellone, Jun Q. Lu, Xin Hua Hu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.

Original languageEnglish
Pages (from-to)521-527
Number of pages7
JournalJournal of Biophotonics
Volume2
Issue number8-9
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Melanoma
Microscopes
Diffraction
microscopes
Imaging techniques
cells
diffraction
Genetic Structures
Polystyrenes
Mie scattering
mice
polystyrene
Theoretical Models
Gels
projection
gels
Light

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Jacobs, K. M., Yang, L. V., Ding, J., Ekpenyong, A., Castellone, R., Lu, J. Q., & Hu, X. H. (2009). Diffraction imaging of spheres and melanoma cells with a microscope objective. Journal of Biophotonics, 2(8-9), 521-527. https://doi.org/10.1002/jbio.200910044

Diffraction imaging of spheres and melanoma cells with a microscope objective. / Jacobs, Kenneth M.; Yang, Li V.; Ding, Junhua; Ekpenyong, Andrew; Castellone, Reid; Lu, Jun Q.; Hu, Xin Hua.

In: Journal of Biophotonics, Vol. 2, No. 8-9, 09.2009, p. 521-527.

Research output: Contribution to journalArticle

Jacobs, KM, Yang, LV, Ding, J, Ekpenyong, A, Castellone, R, Lu, JQ & Hu, XH 2009, 'Diffraction imaging of spheres and melanoma cells with a microscope objective', Journal of Biophotonics, vol. 2, no. 8-9, pp. 521-527. https://doi.org/10.1002/jbio.200910044
Jacobs, Kenneth M. ; Yang, Li V. ; Ding, Junhua ; Ekpenyong, Andrew ; Castellone, Reid ; Lu, Jun Q. ; Hu, Xin Hua. / Diffraction imaging of spheres and melanoma cells with a microscope objective. In: Journal of Biophotonics. 2009 ; Vol. 2, No. 8-9. pp. 521-527.
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