Abstract
Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.
| Original language | English |
|---|---|
| Pages (from-to) | 521-527 |
| Number of pages | 7 |
| Journal | Journal of Biophotonics |
| Volume | 2 |
| Issue number | 8-9 |
| DOIs | |
| State | Published - Sep 2009 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Diffraction imaging of spheres and melanoma cells with a microscope objective. / Jacobs, Kenneth M.; Yang, Li V.; Ding, Junhua; Ekpenyong, Andrew; Castellone, Reid; Lu, Jun Q.; Hu, Xin Hua.
In: Journal of Biophotonics, Vol. 2, No. 8-9, 09.2009, p. 521-527.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diffraction imaging of spheres and melanoma cells with a microscope objective
AU - Jacobs, Kenneth M.
AU - Yang, Li V.
AU - Ding, Junhua
AU - Ekpenyong, Andrew
AU - Castellone, Reid
AU - Lu, Jun Q.
AU - Hu, Xin Hua
PY - 2009/9
Y1 - 2009/9
N2 - Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.
AB - Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.
UR - http://www.scopus.com/inward/record.url?scp=70349701179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349701179&partnerID=8YFLogxK
U2 - 10.1002/jbio.200910044
DO - 10.1002/jbio.200910044
M3 - Article
C2 - 19593764
AN - SCOPUS:70349701179
VL - 2
SP - 521
EP - 527
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 8-9
ER -