Diffraction imaging of spheres and melanoma cells with a microscope objective

Kenneth M. Jacobs; Li V. Yang; Junhua Ding; Andrew E. Ekpenyong; Reid Castellone; Jun Q. Lu; Xin Hua Hu

doi:10.1002/jbio.200910044

Diffraction imaging of spheres and melanoma cells with a microscope objective

Kenneth M. Jacobs, Li V. Yang, Junhua Ding, Andrew E. Ekpenyong, Reid Castellone, Jun Q. Lu, Xin Hua Hu

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.

Original language	English (US)
Pages (from-to)	521-527
Number of pages	7
Journal	Journal of Biophotonics
Volume	2
Issue number	8-9
DOIs	https://doi.org/10.1002/jbio.200910044
State	Published - Sep 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology(all)
Engineering(all)
Physics and Astronomy(all)

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title = "Diffraction imaging of spheres and melanoma cells with a microscope objective",

abstract = "Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.",

author = "Jacobs, {Kenneth M.} and Yang, {Li V.} and Junhua Ding and Ekpenyong, {Andrew E.} and Reid Castellone and Lu, {Jun Q.} and Hu, {Xin Hua}",

year = "2009",

month = sep,

doi = "10.1002/jbio.200910044",

language = "English (US)",

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issn = "1864-063X",

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T1 - Diffraction imaging of spheres and melanoma cells with a microscope objective

AU - Jacobs, Kenneth M.

AU - Yang, Li V.

AU - Ding, Junhua

AU - Ekpenyong, Andrew E.

AU - Castellone, Reid

AU - Lu, Jun Q.

AU - Hu, Xin Hua

PY - 2009/9

Y1 - 2009/9

N2 - Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.

AB - Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.

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JF - Journal of Biophotonics

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Diffraction imaging of spheres and melanoma cells with a microscope objective

Abstract

All Science Journal Classification (ASJC) codes

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