Disruption of gastrulation and oral-aboral ectoderm differentiation in the Lytechinus pictus embryo by a dominant/negative PDGF receptor

Ravi K. Ramachandran, Athula H. Wikramanayake, J. Akif Uzman, Venkatesh Govindarajan, Craig R. Tomlinson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Little is known about the cell signaling involved in forming the body plan of the sea urchin embryo. Previous work suggested that PDGF-like and EGF-like receptor-mediated signaling pathways are involved in gastrulation and spiculogenesis in the Lytechinus pictus embryo. Here we show that expression of the human PDGF receptor-β lacking the cytoplasmic domain disrupted development in a manner consistent with a dominant/negative mechanism. The truncated PDGF receptor-β inhibited gut and spicule formation and differentiation along the oral-aboral axis. The most severely affected embryos arrested at a developmental stage resembling mesenchyme blastula. Coinjection into eggs of RNA encoding the entire human PDGF receptor-β rescued development. The truncated PDGF receptor-β caused the aboral ectoderm-specific genes LpS1 and LpC2 to be repressed while an oral ectoderm-specific gene, Ecto-V, was expressed in all ectoderm cells. The results support the hypothesis that a PDGF-like signaling pathway plays a key role in the intercellular communication required for gastrulation and spiculogenesis, and in cell commitment and differentiation along the oral-aboral axis.

Original languageEnglish
Pages (from-to)2355-2364
Number of pages10
JournalDevelopment
Volume124
Issue number12
StatePublished - Jun 1997
Externally publishedYes

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Lytechinus
Platelet-Derived Growth Factor Receptors
Gastrulation
Ectoderm
Embryonic Structures
Blastula
Sea Urchins
Mesoderm
Epidermal Growth Factor Receptor
Eggs
Genes
Cell Differentiation
RNA

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Cell Biology

Cite this

Disruption of gastrulation and oral-aboral ectoderm differentiation in the Lytechinus pictus embryo by a dominant/negative PDGF receptor. / Ramachandran, Ravi K.; Wikramanayake, Athula H.; Uzman, J. Akif; Govindarajan, Venkatesh; Tomlinson, Craig R.

In: Development, Vol. 124, No. 12, 06.1997, p. 2355-2364.

Research output: Contribution to journalArticle

Ramachandran, Ravi K. ; Wikramanayake, Athula H. ; Uzman, J. Akif ; Govindarajan, Venkatesh ; Tomlinson, Craig R. / Disruption of gastrulation and oral-aboral ectoderm differentiation in the Lytechinus pictus embryo by a dominant/negative PDGF receptor. In: Development. 1997 ; Vol. 124, No. 12. pp. 2355-2364.
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