Disruptive CHD8 mutations define a subtype of autism early in development

Raphael Bernier, Christelle Golzio, Bo Xiong, Holly Stessman, Bradley P. Coe, Osnat Penn, Kali Witherspoon, Jennifer Gerdts, Carl Baker, Anneke T. Vulto-Van Silfhout, Janneke H. Schuurs-Hoeijmakers, Marco Fichera, Paolo Bosco, Serafino Buono, Antonino Alberti, Pinella Failla, Hilde Peeters, Jean Steyaert, Lisenka E L M Vissers, Ludmila FrancescattoHeather C. Mefford, Jill A. Rosenfeld, Trygve Bakken, Brian J. O'Roak, Matthew Pawlus, Randall Moon, Jay Shendure, David G. Amaral, Ed Lein, Julia Rankin, Corrado Romano, Bert B A De Vries, Nicholas Katsanis, Evan E. Eichler

Research output: Contribution to journalArticle

277 Citations (Scopus)

Abstract

Autism spectrum disorder (ASD) is a heterogeneous disease in which efforts to define subtypes behaviorally have met with limited success. Hypothesizing that genetically based subtype identification may prove more productive, we resequenced the ASD-associated gene CHD8 in 3,730 children with developmental delay or ASD. We identified a total of 15 independent mutations; no truncating events were identified in 8,792 controls, including 2,289 unaffected siblings. In addition to a high likelihood of an ASD diagnosis among patients bearing CHD8 mutations, characteristics enriched in this group included macrocephaly, distinct faces, and gastrointestinal complaints. chd8 disruption in zebrafish recapitulates features of the human phenotype, including increased head size as a result of expansion of the forebrain/midbrain and impairment of gastrointestinal motility due to a reduction in postmitotic enteric neurons. Our findings indicate that CHD8 disruptions define a distinct ASD subtype and reveal unexpected comorbidities between brain development and enteric innervation.

Original languageEnglish (US)
Pages (from-to)263-276
Number of pages14
JournalCell
Volume158
Issue number2
DOIs
StatePublished - Jul 17 2014
Externally publishedYes

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Bearings (structural)
Autistic Disorder
Neurons
Brain
Genes
Mutation
Megalencephaly
Gastrointestinal Motility
Zebrafish
Prosencephalon
Mesencephalon
Comorbidity
Siblings
Head
Autism Spectrum Disorder
Phenotype

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bernier, R., Golzio, C., Xiong, B., Stessman, H., Coe, B. P., Penn, O., ... Eichler, E. E. (2014). Disruptive CHD8 mutations define a subtype of autism early in development. Cell, 158(2), 263-276. https://doi.org/10.1016/j.cell.2014.06.017

Disruptive CHD8 mutations define a subtype of autism early in development. / Bernier, Raphael; Golzio, Christelle; Xiong, Bo; Stessman, Holly; Coe, Bradley P.; Penn, Osnat; Witherspoon, Kali; Gerdts, Jennifer; Baker, Carl; Vulto-Van Silfhout, Anneke T.; Schuurs-Hoeijmakers, Janneke H.; Fichera, Marco; Bosco, Paolo; Buono, Serafino; Alberti, Antonino; Failla, Pinella; Peeters, Hilde; Steyaert, Jean; Vissers, Lisenka E L M; Francescatto, Ludmila; Mefford, Heather C.; Rosenfeld, Jill A.; Bakken, Trygve; O'Roak, Brian J.; Pawlus, Matthew; Moon, Randall; Shendure, Jay; Amaral, David G.; Lein, Ed; Rankin, Julia; Romano, Corrado; De Vries, Bert B A; Katsanis, Nicholas; Eichler, Evan E.

In: Cell, Vol. 158, No. 2, 17.07.2014, p. 263-276.

Research output: Contribution to journalArticle

Bernier, R, Golzio, C, Xiong, B, Stessman, H, Coe, BP, Penn, O, Witherspoon, K, Gerdts, J, Baker, C, Vulto-Van Silfhout, AT, Schuurs-Hoeijmakers, JH, Fichera, M, Bosco, P, Buono, S, Alberti, A, Failla, P, Peeters, H, Steyaert, J, Vissers, LELM, Francescatto, L, Mefford, HC, Rosenfeld, JA, Bakken, T, O'Roak, BJ, Pawlus, M, Moon, R, Shendure, J, Amaral, DG, Lein, E, Rankin, J, Romano, C, De Vries, BBA, Katsanis, N & Eichler, EE 2014, 'Disruptive CHD8 mutations define a subtype of autism early in development', Cell, vol. 158, no. 2, pp. 263-276. https://doi.org/10.1016/j.cell.2014.06.017
Bernier, Raphael ; Golzio, Christelle ; Xiong, Bo ; Stessman, Holly ; Coe, Bradley P. ; Penn, Osnat ; Witherspoon, Kali ; Gerdts, Jennifer ; Baker, Carl ; Vulto-Van Silfhout, Anneke T. ; Schuurs-Hoeijmakers, Janneke H. ; Fichera, Marco ; Bosco, Paolo ; Buono, Serafino ; Alberti, Antonino ; Failla, Pinella ; Peeters, Hilde ; Steyaert, Jean ; Vissers, Lisenka E L M ; Francescatto, Ludmila ; Mefford, Heather C. ; Rosenfeld, Jill A. ; Bakken, Trygve ; O'Roak, Brian J. ; Pawlus, Matthew ; Moon, Randall ; Shendure, Jay ; Amaral, David G. ; Lein, Ed ; Rankin, Julia ; Romano, Corrado ; De Vries, Bert B A ; Katsanis, Nicholas ; Eichler, Evan E. / Disruptive CHD8 mutations define a subtype of autism early in development. In: Cell. 2014 ; Vol. 158, No. 2. pp. 263-276.
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AU - O'Roak, Brian J.

AU - Pawlus, Matthew

AU - Moon, Randall

AU - Shendure, Jay

AU - Amaral, David G.

AU - Lein, Ed

AU - Rankin, Julia

AU - Romano, Corrado

AU - De Vries, Bert B A

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