Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells

Kirk Beisel, Sonia Rocha-Sanchez, Sylvia J. Ziegenbein, Ken A. Morris, Chikatoshi Kai, Jun Kawai, Piero Carninci, Yoshihide Hayashizaki, Robin L. Davis

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hair cells express a complement of ion channels, representing shared and distinct channels that confer distinct electrophysiological signatures for each cell. This diversity is generated by the use of alternative splicing in the α subunit, formation of heterotetrameric channels, and combinatorial association with β subunits. These channels are thought to play a role in the tonotopic gradient observed in the mammalian cochlea. Mouse Kcnma1 transcripts, 5′ and 3′ ESTs, and genomic sequences were examined for the utilization of alternative splicing in the mouse transcriptome. Comparative genomic analyses investigated the conservation of KCNMA1 splice sites. Genomes of mouse, rat, human, opossum, chicken, frog and zebrafish established that the exon-intron structure and mechanism of KCNMA1 alternative splicing were highly conserved with 6-7 splice sites being utilized. The murine Kcnma1 utilized 6 out of 7 potential splice sites. RT-PCR experiments using murine gene-specific oligonucleotide primers analyzed the scope and variety of Kcnma1 and Kcnmb1-4 expression profiles in the cochlea and inner ear hair cells. In the cochlea splice variants were present representing sites 3, 4, 6, and 7, while site 1 was insertionless and site 2 utilized only exon 10. However, site 5 was not present. Detection of KCNMA1 transcripts and protein exhibited a quantitative longitudinal gradient with a reciprocal gradient found between inner and outer hair cells. Differential expression was also observed in the usage of the long form of the carboxy-terminus tail. These results suggest that a diversity of splice variants exist in rodent cochlear hair cells and this diversity is similar to that observed for non-mammalian vertebrate hair cells, such as chicken and turtle.

Original languageEnglish
Pages (from-to)11-23
Number of pages13
JournalGene
Volume386
Issue number1-2
DOIs
StatePublished - Jan 15 2007

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Inner Auditory Hair Cells
Calcium-Activated Potassium Channels
Cochlea
Alternative Splicing
Inner Ear
Outer Auditory Hair Cells
Chickens
Exons
Auditory Hair Cells
Opossums
Turtles
DNA Primers
Expressed Sequence Tags
Zebrafish
Ion Channels
Transcriptome
Anura
Introns
Vertebrates
Tail

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Beisel, K., Rocha-Sanchez, S., Ziegenbein, S. J., Morris, K. A., Kai, C., Kawai, J., ... Davis, R. L. (2007). Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells. Gene, 386(1-2), 11-23. https://doi.org/10.1016/j.gene.2006.07.023

Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells. / Beisel, Kirk; Rocha-Sanchez, Sonia; Ziegenbein, Sylvia J.; Morris, Ken A.; Kai, Chikatoshi; Kawai, Jun; Carninci, Piero; Hayashizaki, Yoshihide; Davis, Robin L.

In: Gene, Vol. 386, No. 1-2, 15.01.2007, p. 11-23.

Research output: Contribution to journalArticle

Beisel, K, Rocha-Sanchez, S, Ziegenbein, SJ, Morris, KA, Kai, C, Kawai, J, Carninci, P, Hayashizaki, Y & Davis, RL 2007, 'Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells', Gene, vol. 386, no. 1-2, pp. 11-23. https://doi.org/10.1016/j.gene.2006.07.023
Beisel, Kirk ; Rocha-Sanchez, Sonia ; Ziegenbein, Sylvia J. ; Morris, Ken A. ; Kai, Chikatoshi ; Kawai, Jun ; Carninci, Piero ; Hayashizaki, Yoshihide ; Davis, Robin L. / Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells. In: Gene. 2007 ; Vol. 386, No. 1-2. pp. 11-23.
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