DNA bending by GCN4 mutants bearing cationic residues

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Transcription activation is thought to require DNA bending to promote the interaction of upstream activators and the basal transcription machinery. Previous experiments have shown that some members of the bZIP family of DNA binding proteins bend DNA, while others do not. We are exploring the possibility that electrostatic effects play a role in these differences. The yeast bZIP transcription factor GCN4 does not induce DNA bending in vitro. Substitution of basic residues for three neutral amino acids of GCN4 confers the ability to bend DNA. This result is consistent with a model of induced DNA bending wherein excess positive charge in proximity to one face of the double helix neutralizes local phosphate diester anions resulting in a laterally asymmetric charge distribution along the DNA. Previous data suggest that such an unbalanced charge distribution results in collapse of the DNA toward the neutralized surface. Interpretations of the present data are discussed. Our result supports the hypothesis that electrostatic interactions can play a key role in DNA bending by bZIP proteins.

Original languageEnglish
Pages (from-to)10026-10032
Number of pages7
JournalBiochemistry
Volume36
Issue number33
DOIs
StatePublished - Aug 19 1997
Externally publishedYes

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Bearings (structural)
DNA
Basic-Leucine Zipper Transcription Factors
Charge distribution
Transcription
Static Electricity
Neutral Amino Acids
Aptitude
DNA-Binding Proteins
Coulomb interactions
Yeast
Transcriptional Activation
Machinery
Anions
Electrostatics
Substitution reactions
Yeasts
Chemical activation
Phosphates

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

DNA bending by GCN4 mutants bearing cationic residues. / Strauss-Soukup, Juliane K.; Maher, L. J.

In: Biochemistry, Vol. 36, No. 33, 19.08.1997, p. 10026-10032.

Research output: Contribution to journalArticle

Strauss-Soukup, Juliane K. ; Maher, L. J. / DNA bending by GCN4 mutants bearing cationic residues. In: Biochemistry. 1997 ; Vol. 36, No. 33. pp. 10026-10032.
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