DNAzymes for amine and peptide lysine acylation

Tianjiong Yao, Jack J. Przybyla, Peter Yeh, Austin M. Woodard, Hannah J. Nilsson, Benjamin M. Brandsen, Scott K. Silverman

Research output: Contribution to journalArticlepeer-review

Abstract

DNAzymes were previously identified by in vitro selection for a variety of chemical reactions, including several biologically relevant peptide modifications. However, finding DNAzymes for peptide lysine acylation is a substantial challenge. By using suitably reactive aryl ester acyl donors as the electrophiles, here we used in vitro selection to identify DNAzymes that acylate amines, including lysine side chains of DNA-anchored peptides. Some of the DNAzymes can transfer a small glutaryl group to an amino group. These results expand the scope of DNAzyme catalysis and suggest the future broader applicability of DNAzymes for sequence-selective lysine acylation of peptide and protein substrates. This journal is

Original languageEnglish (US)
Pages (from-to)171-181
Number of pages11
JournalOrganic and Biomolecular Chemistry
Volume19
Issue number1
DOIs
StatePublished - Jan 7 2021
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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