Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: Role of arachidonic acid metabolites

Catherine A. Opere; Wei Dong Zheng; Jifan Huang; Adeniran Adewale; Michael Kruglet; Sunny E. Ohia

doi:10.1007/s11064-004-9694-3

Dual effect of isoprostanes on the release of [³H]D-aspartate from isolated bovine retinae: Role of arachidonic acid metabolites

Catherine A. Opere, Wei Dong Zheng, Jifan Huang, Adeniran Adewale, Michael Kruglet, Sunny E. Ohia

Department of Pharmacy Sciences

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The effect of 8-isoprostanes on potassium (K⁺)-depolarization- evoked release of [³H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K ⁺-evoked release of [³H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF_2α (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 μM) enhanced K⁺-induced [³H]D-aspartate overflow. The excitatory effect of 8-isoPGF_2α was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 μM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF _2α (1-30 μM) on [³H]D-aspartate release that was attenuated by AH 6809 (10 μM). In conclusion, 8-isoPGF_2α exhibits a dual regulatory effect on K⁺-induced [³H]D- aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF_2α is due to the activation of EP₁/EP ₂ receptors while the excitatory effects are due to the activation of thromboxane receptors.

Original language	English (US)
Pages (from-to)	129-137
Number of pages	9
Journal	Neurochemical Research
Volume	30
Issue number	1
DOIs	https://doi.org/10.1007/s11064-004-9694-3
State	Published - Jan 2005

All Science Journal Classification (ASJC) codes

Biochemistry
Cellular and Molecular Neuroscience

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@article{eed1a0a93b2644f59a1a239c43aafa6e,

title = "Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: Role of arachidonic acid metabolites",

abstract = "The effect of 8-isoprostanes on potassium (K+)-depolarization- evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K +-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF2α (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 μM) enhanced K+-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF2α was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 μM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF 2α (1-30 μM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 μM). In conclusion, 8-isoPGF2α exhibits a dual regulatory effect on K+-induced [3H]D- aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF2α is due to the activation of EP1/EP 2 receptors while the excitatory effects are due to the activation of thromboxane receptors.",

author = "Opere, {Catherine A.} and Zheng, {Wei Dong} and Jifan Huang and Adeniran Adewale and Michael Kruglet and Ohia, {Sunny E.}",

note = "Funding Information: The authors thank J.F. O{\textquoteright}neill Packing Company, Omaha and All American Meats, Inc., Omaha for their generous donation of cow eyeballs. This project was supported by Health Future Foundation Faculty Development Award and National Institutes of Health grant EY13967-01A1 to CAO.",

year = "2005",

month = jan,

doi = "10.1007/s11064-004-9694-3",

language = "English (US)",

volume = "30",

pages = "129--137",

journal = "Neurochemical Research",

issn = "0364-3190",

publisher = "Springer New York",

number = "1",

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TY - JOUR

T1 - Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae

T2 - Role of arachidonic acid metabolites

AU - Opere, Catherine A.

AU - Zheng, Wei Dong

AU - Huang, Jifan

AU - Adewale, Adeniran

AU - Kruglet, Michael

AU - Ohia, Sunny E.

N1 - Funding Information: The authors thank J.F. O’neill Packing Company, Omaha and All American Meats, Inc., Omaha for their generous donation of cow eyeballs. This project was supported by Health Future Foundation Faculty Development Award and National Institutes of Health grant EY13967-01A1 to CAO.

PY - 2005/1

Y1 - 2005/1

N2 - The effect of 8-isoprostanes on potassium (K+)-depolarization- evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K +-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF2α (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 μM) enhanced K+-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF2α was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 μM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF 2α (1-30 μM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 μM). In conclusion, 8-isoPGF2α exhibits a dual regulatory effect on K+-induced [3H]D- aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF2α is due to the activation of EP1/EP 2 receptors while the excitatory effects are due to the activation of thromboxane receptors.

AB - The effect of 8-isoprostanes on potassium (K+)-depolarization- evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K +-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF2α (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 μM) enhanced K+-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF2α was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 μM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF 2α (1-30 μM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 μM). In conclusion, 8-isoPGF2α exhibits a dual regulatory effect on K+-induced [3H]D- aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF2α is due to the activation of EP1/EP 2 receptors while the excitatory effects are due to the activation of thromboxane receptors.

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JF - Neurochemical Research

SN - 0364-3190

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