Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: Role of arachidonic acid metabolites

Catherine A. Opere, Wei Dong Zheng, Jifan Huang, Adeniran Adewale, Michael Kruglet, Sunny E. Ohia

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The effect of 8-isoprostanes on potassium (K+)-depolarization- evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K +-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 μM) enhanced K+-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 μM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF (1-30 μM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 μM). In conclusion, 8-isoPGF exhibits a dual regulatory effect on K+-induced [3H]D- aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF is due to the activation of EP1/EP 2 receptors while the excitatory effects are due to the activation of thromboxane receptors.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalNeurochemical Research
Issue number1
StatePublished - Jan 1 2005


All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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