Abstract
A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH2CH 2O)2-CH2CH2NH-biotin (where X = N=C=S or NHCOCH2Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.
Original language | English (US) |
---|---|
Pages (from-to) | 201-204 |
Number of pages | 4 |
Journal | Bioconjugate Chemistry |
Volume | 20 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2009 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry