Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors

Jane V. Aldrich, Vivek Kumar, Thomas F. Murray, Wei Guang, Jia Bei Wang

Research output: Contribution to journal › Article

Abstract

A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH2CH 2O)2-CH2CH2NH-biotin (where X = N=C=S or NHCOCH2Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalBioconjugate Chemistry
Volume20
Issue number2
DOIs
StatePublished - Feb 2009

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phenylalanylphenylalanine
Affinity Labels
Opioid Receptors
Biotin
Peptides
Labels
Derivatives
Solid-Phase Synthesis Techniques
Narcotic Antagonists
Polyacrylamide Gel Electrophoresis
2-tyrosyl-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Dual labeled peptides as tools to study receptors : Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors. / Aldrich, Jane V.; Kumar, Vivek; Murray, Thomas F.; Guang, Wei; Wang, Jia Bei.

In: Bioconjugate Chemistry, Vol. 20, No. 2, 02.2009, p. 201-204.

Research output: Contribution to journal › Article

Aldrich, JV, Kumar, V, Murray, TF, Guang, W & Wang, JB 2009, 'Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors', Bioconjugate Chemistry, vol. 20, no. 2, pp. 201-204. https://doi.org/10.1021/bc800420t
Aldrich JV, Kumar V, Murray TF, Guang W, Wang JB. Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors. Bioconjugate Chemistry. 2009 Feb;20(2):201-204. https://doi.org/10.1021/bc800420t
Aldrich, Jane V. ; Kumar, Vivek ; Murray, Thomas F. ; Guang, Wei ; Wang, Jia Bei. / Dual labeled peptides as tools to study receptors : Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors. In: Bioconjugate Chemistry. 2009 ; Vol. 20, No. 2. pp. 201-204.
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