Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors

Jane V. Aldrich; Vivek Kumar; Thomas F. Murray; Wei Guang; Jia Bei Wang

doi:10.1021/bc800420t

Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors

Jane V. Aldrich, Vivek Kumar, Thomas F. Murray, Wei Guang, Jia Bei Wang

Department of Pharmacology and Neuroscience

Research output: Contribution to journal › Article

Abstract

A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH₂CH ₂O)₂-CH₂CH₂NH-biotin (where X = N=C=S or NHCOCH₂Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.

Original language	English (US)
Pages (from-to)	201-204
Number of pages	4
Journal	Bioconjugate Chemistry
Volume	20
Issue number	2
DOIs	https://doi.org/10.1021/bc800420t
State	Published - Feb 1 2009

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

Access to Document

10.1021/bc800420t

Fingerprint Dive into the research topics of 'Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors'. Together they form a unique fingerprint.

Cite this

Aldrich, J. V., Kumar, V., Murray, T. F., Guang, W., & Wang, J. B. (2009). Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors. Bioconjugate Chemistry, 20(2), 201-204. https://doi.org/10.1021/bc800420t

Dual labeled peptides as tools to study receptors : Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors. / Aldrich, Jane V.; Kumar, Vivek; Murray, Thomas F.; Guang, Wei; Wang, Jia Bei.

In: Bioconjugate Chemistry, Vol. 20, No. 2, 01.02.2009, p. 201-204.

Research output: Contribution to journal › Article

@article{2df7c462bb7b4c42b5e21e860e5718c3,

title = "Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors",

abstract = "A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH2CH 2O)2-CH2CH2NH-biotin (where X = N=C=S or NHCOCH2Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.",

author = "Aldrich, {Jane V.} and Vivek Kumar and Murray, {Thomas F.} and Wei Guang and Wang, {Jia Bei}",

year = "2009",

month = feb,

day = "1",

doi = "10.1021/bc800420t",

language = "English (US)",

volume = "20",

pages = "201--204",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - Dual labeled peptides as tools to study receptors

T2 - Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors

AU - Aldrich, Jane V.

AU - Kumar, Vivek

AU - Murray, Thomas F.

AU - Guang, Wei

AU - Wang, Jia Bei

PY - 2009/2/1

Y1 - 2009/2/1

N2 - A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH2CH 2O)2-CH2CH2NH-biotin (where X = N=C=S or NHCOCH2Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.

AB - A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH2CH 2O)2-CH2CH2NH-biotin (where X = N=C=S or NHCOCH2Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.

UR - http://www.scopus.com/inward/record.url?scp=62549108152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62549108152&partnerID=8YFLogxK

U2 - 10.1021/bc800420t

DO - 10.1021/bc800420t

M3 - Article

C2 - 19175314

AN - SCOPUS:62549108152

VL - 20

SP - 201

EP - 204

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 2

ER -