Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors

Jane V. Aldrich; Vivek Kumar; Thomas F. Murray; Wei Guang; Jia Bei Wang

doi:10.1021/bc800420t

Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors

Jane V. Aldrich, Vivek Kumar, Thomas F. Murray, Wei Guang, Jia Bei Wang

Department of Pharmacology and Neuroscience

Research output: Contribution to journal › Article › peer-review

Abstract

A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH₂CH ₂O)₂-CH₂CH₂NH-biotin (where X = N=C=S or NHCOCH₂Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.

Original language	English (US)
Pages (from-to)	201-204
Number of pages	4
Journal	Bioconjugate Chemistry
Volume	20
Issue number	2
DOIs	https://doi.org/10.1021/bc800420t
State	Published - Feb 2009

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/bc800420t

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Dual labeled peptides as tools to study receptors : Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors. / Aldrich, Jane V.; Kumar, Vivek; Murray, Thomas F.; Guang, Wei; Wang, Jia Bei.

In: Bioconjugate Chemistry, Vol. 20, No. 2, 02.2009, p. 201-204.

Research output: Contribution to journal › Article › peer-review

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Dual labeled peptides as tools to study receptors: Nanomolar affinity derivatives of TIPP (Tyr-Tic-Phe-Phe) containing an affinity label and biotin as probes of δ opioid receptors

Abstract

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