TY - CHAP
T1 - Early steps of V(D)J rearrangement
T2 - Insights from biochemical studies of RAG-RSS complexes
AU - Swanson, Patrick
AU - Kumar, Sushi
AU - Raval, Prafulla
PY - 2009
Y1 - 2009
N2 - V:(D)J recombinationis initiated by the synapsis and cleavage of a complementary(12123) pair of recombination signalsequences (RSSs) by the RAG1 and RAG2 proteins. Our understanding of these processes has been greadyaided by the development of in vitro biochemicalassays of RAG binding and cleavage activity. Accumulating evidencesuggests that synapticcomplexassembly occursin a step-wise manner and that the RAG proteins catalyze RSS cleavage by mechanisms similar to those used by bacterial transposases. In this chapter we will reviewthe molecularmechanisms of RAG synapticcomplexassembly and 12123-regulated RSS cleavage, focusingon recent advances that shed newlight on theseprocesses.
AB - V:(D)J recombinationis initiated by the synapsis and cleavage of a complementary(12123) pair of recombination signalsequences (RSSs) by the RAG1 and RAG2 proteins. Our understanding of these processes has been greadyaided by the development of in vitro biochemicalassays of RAG binding and cleavage activity. Accumulating evidencesuggests that synapticcomplexassembly occursin a step-wise manner and that the RAG proteins catalyze RSS cleavage by mechanisms similar to those used by bacterial transposases. In this chapter we will reviewthe molecularmechanisms of RAG synapticcomplexassembly and 12123-regulated RSS cleavage, focusingon recent advances that shed newlight on theseprocesses.
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U2 - 10.1007/978-1-4419-0296-2_1
DO - 10.1007/978-1-4419-0296-2_1
M3 - Chapter
C2 - 19731797
AN - SCOPUS:70349572500
SN - 9781441902955
VL - 650
T3 - Advances in Experimental Medicine and Biology
SP - 1
EP - 15
BT - V(D)J Recombination
ER -