Ectodermal syndecan-2 mediates left-right axis formation in migrating mesoderm as a cell-nonautonomous Vg1 cofactor

Kenneth L. Kramer; H. Joseph Yost

doi:10.1016/S1534-5807(01)00107-1

Ectodermal syndecan-2 mediates left-right axis formation in migrating mesoderm as a cell-nonautonomous Vg1 cofactor

Kenneth L. Kramer, H. Joseph Yost

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

Heparan sulfate proteoglycans expressed on the Xenopus animal cap ectoderm have been implicated in transmitting left-right information to heart and gut primordia. We report here that syndecan-2 functions in the ectoderm to mediate cardiac and visceral situs, upstream of known asymmetrically expressed genes but independently of its ability to mediate fibronectin fibrillogenesis. Left-right development is dependent on a distinct subset of glycosaminoglycan attachment sites on syndecan-2. A novel in vivo approach with enterokinase demonstrates that syndecan-2 functions in left-right patterning during early gastrulation. We describe a cell-nonautonomous role for ectodermal syndecan-2 in transmitting left-right information to migrating mesoderm. The results further suggest that this function may be related to the transduction of Vg1-related signals.

Original language	English (US)
Pages (from-to)	115-124
Number of pages	10
Journal	Developmental Cell
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/S1534-5807(01)00107-1
State	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Developmental Biology
Cell Biology

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10.1016/S1534-5807(01)00107-1

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title = "Ectodermal syndecan-2 mediates left-right axis formation in migrating mesoderm as a cell-nonautonomous Vg1 cofactor",

abstract = "Heparan sulfate proteoglycans expressed on the Xenopus animal cap ectoderm have been implicated in transmitting left-right information to heart and gut primordia. We report here that syndecan-2 functions in the ectoderm to mediate cardiac and visceral situs, upstream of known asymmetrically expressed genes but independently of its ability to mediate fibronectin fibrillogenesis. Left-right development is dependent on a distinct subset of glycosaminoglycan attachment sites on syndecan-2. A novel in vivo approach with enterokinase demonstrates that syndecan-2 functions in left-right patterning during early gastrulation. We describe a cell-nonautonomous role for ectodermal syndecan-2 in transmitting left-right information to migrating mesoderm. The results further suggest that this function may be related to the transduction of Vg1-related signals.",

author = "Kramer, {Kenneth L.} and Yost, {H. Joseph}",

note = "Funding Information: We thank J. Finney and C. Zundel for excellent technical support, J. Couchman for helpful suggestions, C. Wright, J. Izpisua-Belmonte, G. Thomsen, L. Dale, S. Sokol, and D. DeSimone for reagents, A. Ramsdell for communicating results prior to publication, and B. Bisgrove, W. Branford, C-B. Chien, M. Condic, J. Essner, and D. Grunwald for constructive comments. K.L.K. is supported by a National Research Service Award from the National Heart, Lung and Blood Institute, and H.J.Y. is an Established Investigator of the American Heart Association. This work was supported by a NIH SCOR grant.",

year = "2002",

doi = "10.1016/S1534-5807(01)00107-1",

language = "English (US)",

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pages = "115--124",

journal = "Developmental Cell",

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AU - Kramer, Kenneth L.

AU - Yost, H. Joseph

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PY - 2002

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N2 - Heparan sulfate proteoglycans expressed on the Xenopus animal cap ectoderm have been implicated in transmitting left-right information to heart and gut primordia. We report here that syndecan-2 functions in the ectoderm to mediate cardiac and visceral situs, upstream of known asymmetrically expressed genes but independently of its ability to mediate fibronectin fibrillogenesis. Left-right development is dependent on a distinct subset of glycosaminoglycan attachment sites on syndecan-2. A novel in vivo approach with enterokinase demonstrates that syndecan-2 functions in left-right patterning during early gastrulation. We describe a cell-nonautonomous role for ectodermal syndecan-2 in transmitting left-right information to migrating mesoderm. The results further suggest that this function may be related to the transduction of Vg1-related signals.

AB - Heparan sulfate proteoglycans expressed on the Xenopus animal cap ectoderm have been implicated in transmitting left-right information to heart and gut primordia. We report here that syndecan-2 functions in the ectoderm to mediate cardiac and visceral situs, upstream of known asymmetrically expressed genes but independently of its ability to mediate fibronectin fibrillogenesis. Left-right development is dependent on a distinct subset of glycosaminoglycan attachment sites on syndecan-2. A novel in vivo approach with enterokinase demonstrates that syndecan-2 functions in left-right patterning during early gastrulation. We describe a cell-nonautonomous role for ectodermal syndecan-2 in transmitting left-right information to migrating mesoderm. The results further suggest that this function may be related to the transduction of Vg1-related signals.

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Ectodermal syndecan-2 mediates left-right axis formation in migrating mesoderm as a cell-nonautonomous Vg1 cofactor

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