Effect of acetaminophen on the leukocyte-labeling efficiency of indium oxine In 111

Samuel C. Augustine, R. F. Schmelter, K. L. Nelson, R. J. Petersen, M. A. Qualfe

Research output: Contribution to journalArticle

Abstract

The effect of acetaminophen on the labeling efficiency of leukocytes with indium oxine In 111 was studied. A blood sample was obtained from eight healthy men before and after they received acetaminophen 650 mg every four hours for 24 hours. After dividing the plasma from each sample into three portions, leukocytes were separated and labeled with indium oxine In 111. In an in vitro study, 200 ml of blood was obtained from one of the men, and the plasma was separated into four portions. Acetaminophen in 95% ethanol was added to three of the plasma fractions to produce acetaminophen concentrations of 4, 20, and 100 μg/ml; ethanol was added to the fourth fraction as a control. Each plasma fraction was then subdivided into three aliquots, and leukocytes were labeled as in the in vivo study. Mean leukocyte labeling efficiencies in both studies were calculated from the ratios of leukocyte radioactivity to initial radioactivity in the samples, expressed as percentages. Leukocyte labeling efficiencies before acetaminophen administration ranged from 79 to 85%; after administration, labeling efficiencies ranged from 70 to 87%. No significant differences in mean labeling efficiency before and after acetaminophen administration were noted in any of the subjects. Leukocyte labeling efficiencies in all in vitro plasma fractions were reduced, ranging from 54 to 63%, but no significant differences in labeling efficiency between any of the plasma fractions were found. Using the labeling procedures in this study, exposure of leukocytes from healthy men to acetaminophen in vivo or in vitro does not affect labeling efficiency with indium oxine In 111.

Original languageEnglish
Pages (from-to)1965-1967
Number of pages3
JournalAmerican Journal of Hospital Pharmacy
Volume40
Issue number11
StatePublished - 1983
Externally publishedYes

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Acetaminophen
Leukocytes
Radioactivity
Ethanol
indium oxine
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Leadership and Management

Cite this

Augustine, S. C., Schmelter, R. F., Nelson, K. L., Petersen, R. J., & Qualfe, M. A. (1983). Effect of acetaminophen on the leukocyte-labeling efficiency of indium oxine In 111. American Journal of Hospital Pharmacy, 40(11), 1965-1967.

Effect of acetaminophen on the leukocyte-labeling efficiency of indium oxine In 111. / Augustine, Samuel C.; Schmelter, R. F.; Nelson, K. L.; Petersen, R. J.; Qualfe, M. A.

In: American Journal of Hospital Pharmacy, Vol. 40, No. 11, 1983, p. 1965-1967.

Research output: Contribution to journalArticle

Augustine, SC, Schmelter, RF, Nelson, KL, Petersen, RJ & Qualfe, MA 1983, 'Effect of acetaminophen on the leukocyte-labeling efficiency of indium oxine In 111', American Journal of Hospital Pharmacy, vol. 40, no. 11, pp. 1965-1967.
Augustine, Samuel C. ; Schmelter, R. F. ; Nelson, K. L. ; Petersen, R. J. ; Qualfe, M. A. / Effect of acetaminophen on the leukocyte-labeling efficiency of indium oxine In 111. In: American Journal of Hospital Pharmacy. 1983 ; Vol. 40, No. 11. pp. 1965-1967.
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abstract = "The effect of acetaminophen on the labeling efficiency of leukocytes with indium oxine In 111 was studied. A blood sample was obtained from eight healthy men before and after they received acetaminophen 650 mg every four hours for 24 hours. After dividing the plasma from each sample into three portions, leukocytes were separated and labeled with indium oxine In 111. In an in vitro study, 200 ml of blood was obtained from one of the men, and the plasma was separated into four portions. Acetaminophen in 95{\%} ethanol was added to three of the plasma fractions to produce acetaminophen concentrations of 4, 20, and 100 μg/ml; ethanol was added to the fourth fraction as a control. Each plasma fraction was then subdivided into three aliquots, and leukocytes were labeled as in the in vivo study. Mean leukocyte labeling efficiencies in both studies were calculated from the ratios of leukocyte radioactivity to initial radioactivity in the samples, expressed as percentages. Leukocyte labeling efficiencies before acetaminophen administration ranged from 79 to 85{\%}; after administration, labeling efficiencies ranged from 70 to 87{\%}. No significant differences in mean labeling efficiency before and after acetaminophen administration were noted in any of the subjects. Leukocyte labeling efficiencies in all in vitro plasma fractions were reduced, ranging from 54 to 63{\%}, but no significant differences in labeling efficiency between any of the plasma fractions were found. Using the labeling procedures in this study, exposure of leukocytes from healthy men to acetaminophen in vivo or in vitro does not affect labeling efficiency with indium oxine In 111.",
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