Effect of base composition on DNA bending by phosphate neutralization

Juliane K. Strauss-Soukup, Paula D. Rodrigues, L. James Maher

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Of the many forces involved in DNA bending by proteins, we have focused on the possible role of asymmetric phosphate neutralization due to interactions between the negatively charged phosphate backbone of duplex DNA and cationic amino acids of an approaching protein. The resulting unbalanced charge distribution along the duplex DNA is thought to induce the double helix to collapse toward the neutralized surface. Previous work has confirmed that DNA bending (~ 20.7 ± 4°) is induced by asymmetric incorporation of six uncharged racemic methylphosphonate analogs partially neutralizing one face of GC-rich duplex DNA. We have now analyzed DNA duplexes with similar patches of methylphosphonate linkages in an AT-rich sequence context and again observe bending toward the neutralized face, to an extent (20 ± 0.6°) comparable to that observed for neutral patches in GC-rich DNA. The similar induced bend angles in AT-rich and GC-rich contexts does not reveal increased flexibility in AT-rich sequences, or a particular propensity of A-T base pairs to roll toward the minor groove in the tested sequences.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalBiophysical Chemistry
Volume72
Issue number3
DOIs
StatePublished - Jun 9 1998
Externally publishedYes

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Base Composition
phosphates
deoxyribonucleic acid
Phosphates
DNA
Chemical analysis
AT Rich Sequence
proteins
Charge distribution
linkages
Base Pairing
grooves
helices
charge distribution
amino acids
flexibility
Proteins
analogs
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Biophysics

Cite this

Effect of base composition on DNA bending by phosphate neutralization. / Strauss-Soukup, Juliane K.; D. Rodrigues, Paula; Maher, L. James.

In: Biophysical Chemistry, Vol. 72, No. 3, 09.06.1998, p. 297-306.

Research output: Contribution to journalArticle

Strauss-Soukup, Juliane K. ; D. Rodrigues, Paula ; Maher, L. James. / Effect of base composition on DNA bending by phosphate neutralization. In: Biophysical Chemistry. 1998 ; Vol. 72, No. 3. pp. 297-306.
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