Effect of diabetes on vascular smooth muscle function in normotensive and spontaneously hypertensive rat mesenteric artery

Devendra K. Agrawal, J. H. McNeill

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Abstract

We have examined the functional responses to α-adrenoceptor agonists (α1-selective: methoxamine and phenylephrine; α2-selective: clonidine and B-HT 920; non-selective: norepinephrine, serotonin and K+ in ring segments of mesenteric artery of control and streptozotocin-induced diabetic spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. Systolic blood pressure and contractile responses were examined after 12 weeks of diabetes. There was no significant change in the diabetic WKY rats as compared with the control WKY rats. However, diabetic SHR had significantly less hypertension than control SHR. Responses to serotonin and α2-adrenoceptor agonists were augmented significantly in arteries from control SHR animals as compared with vessels from WKY animals. There was no significant difference in the force of contraction generated by other agonists in both nondiabetic groups. Responses to all agonists in WKY diabetic and to methoxamine and K+ in SHR diabetic arteries were increased as compared with their respective controls. ED50 values for each agonist were similar in all groups. Indomethacin (5 μM) shifted the dose-response curve to norepinephrine to the right in arteries from all groups of animals. However, in the diabetic SHR and WKY, there was a significant reduction in norepinephrine maximum response. Nifedipine was more potent in inhibiting the contraction to K+ and serotonin in WKY diabetic arteries as compared with WKY controls. However, nifedipine inhibited the responses to all agonists with equal potency in the control and diabetic SHR vessels. These results suggest the involvement of α2-adrenoceptors and serotonin receptors in the development and (or) the maintenance of hypertension. Furthermore, the hyperresponsiveness of the diabetic vessels could be due to an alteration in the postreceptor excitation-contraction coupling, possibly involving prostaglandin and an increased activity of calcium channels.

Original languageEnglish
Pages (from-to)2274-2280
Number of pages7
JournalCanadian Journal of Physiology and Pharmacology
Volume65
Issue number11
StatePublished - 1988
Externally publishedYes

Fingerprint

Mesenteric Arteries
Inbred SHR Rats
Vascular Smooth Muscle
Inbred WKY Rats
Arteries
Adrenergic Receptors
Methoxamine
Serotonin
Norepinephrine
Nifedipine
Serotonin 5-HT2 Receptors
Blood Pressure
Hypertension
Excitation Contraction Coupling
Clonidine
Phenylephrine
Calcium Channels
Streptozocin
Indomethacin
Prostaglandins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology

Cite this

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abstract = "We have examined the functional responses to α-adrenoceptor agonists (α1-selective: methoxamine and phenylephrine; α2-selective: clonidine and B-HT 920; non-selective: norepinephrine, serotonin and K+ in ring segments of mesenteric artery of control and streptozotocin-induced diabetic spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. Systolic blood pressure and contractile responses were examined after 12 weeks of diabetes. There was no significant change in the diabetic WKY rats as compared with the control WKY rats. However, diabetic SHR had significantly less hypertension than control SHR. Responses to serotonin and α2-adrenoceptor agonists were augmented significantly in arteries from control SHR animals as compared with vessels from WKY animals. There was no significant difference in the force of contraction generated by other agonists in both nondiabetic groups. Responses to all agonists in WKY diabetic and to methoxamine and K+ in SHR diabetic arteries were increased as compared with their respective controls. ED50 values for each agonist were similar in all groups. Indomethacin (5 μM) shifted the dose-response curve to norepinephrine to the right in arteries from all groups of animals. However, in the diabetic SHR and WKY, there was a significant reduction in norepinephrine maximum response. Nifedipine was more potent in inhibiting the contraction to K+ and serotonin in WKY diabetic arteries as compared with WKY controls. However, nifedipine inhibited the responses to all agonists with equal potency in the control and diabetic SHR vessels. These results suggest the involvement of α2-adrenoceptors and serotonin receptors in the development and (or) the maintenance of hypertension. Furthermore, the hyperresponsiveness of the diabetic vessels could be due to an alteration in the postreceptor excitation-contraction coupling, possibly involving prostaglandin and an increased activity of calcium channels.",
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