Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase

L. H. Wang, Yaping Tu, X. Y. Yang, Z. C. Tsui, F. Y. Yang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Trace amounts of gangliosides were found in rabbit skeletal muscle sarcoplasmic reticulum and their main part was shown, by high performance thin layer chromatography, to be GM3. Addition of GM3 to the soybean phospholipids used for reconstitution of proteoliposomes markedly increased ATP hydrolysis as well as Ca2+ uptake activity of sarcoplasmic reticulum Ca2+-ATPase incorporated into the proteoliposomes. Conformation changes of Ca2+-ATPase induced by GM3 were also observed by intrinsic fluorescence and circular dichroism measurements.

Original languageEnglish
Pages (from-to)128-130
Number of pages3
JournalFEBS Letters
Volume388
Issue number2-3
DOIs
StatePublished - Jun 17 1996
Externally publishedYes

Fingerprint

G(M3) Ganglioside
Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Conformations
Thin layer chromatography
Gangliosides
Thin Layer Chromatography
Circular Dichroism
Soybeans
Muscle
Hydrolysis
Phospholipids
Skeletal Muscle
Adenosine Triphosphate
Fluorescence
Rabbits
proteoliposomes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase. / Wang, L. H.; Tu, Yaping; Yang, X. Y.; Tsui, Z. C.; Yang, F. Y.

In: FEBS Letters, Vol. 388, No. 2-3, 17.06.1996, p. 128-130.

Research output: Contribution to journalArticle

Wang, L. H. ; Tu, Yaping ; Yang, X. Y. ; Tsui, Z. C. ; Yang, F. Y. / Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase. In: FEBS Letters. 1996 ; Vol. 388, No. 2-3. pp. 128-130.
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