Effect of hydrogen sulfide donors on intraocular pressure in rabbits

Ankita Salvi, Pratik Bankhele, Jamal Jamil, Madhura Kulkarni Chitnis, Ya Fatou Njie-Mbye, Sunny E. Ohia, Catherine A. Opere

Research output: Contribution to journalArticle

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Abstract

Purpose: In this study, we investigated the effect of a slow-releasing hydrogen sulfide (H2S) donor, GYY 4137, on intraocular pressure (IOP) in normotensive rabbits. Furthermore, we compared the IOP-lowering action of GYY 4137 with those elicited by other H2S-producing compounds, l-cysteine and ACS67 (a hybrid compound of latanoprost with an H2S-releasing moiety). Methods: IOP was measured in New Zealand normotensive male albino rabbits using a pneumatonometer (model 30 classic; Reichert Ophthalmic Instruments, Depew, NY). At 0 h, 50 μL of test compounds were applied topically to 1 eye of each animal, while the contralateral eye received the same quantity of vehicle (saline). IOP was measured hourly until baseline IOP readings were attained and animal eyes monitored for potential side effects (i.e., tearing, hyperemia). Results: GYY 4137 (0.1%-2%) produced a dose-dependent decrease in IOP reaching a maximum of 27.8% ± 3.14% (n = 5) after 6 h. Interestingly, a significant contralateral effect was observed in vehicle-treated controls eyes at all doses tested. l-cysteine (5%) and ACS67 (0.005%) also elicited a significant (P <0.01) decrease in IOP that achieved a maximum of 28.84% ± 1.53% (n = 5) and 23.27% ± 0.51% (n = 5), respectively, after 3 h. All 3 H2S-producing compounds also caused a significant contralateral effect in vehicle-treated control eyes. Conclusion: We conclude that GYY 4137 and other H2S-producing donors can reduce IOP in normotensive rabbits. However, the profile of IOP-lowering action of GYY 4137 was different from the other H2S donors affirming its ability to act as a slow-releasing gas donor.

Original languageEnglish
Pages (from-to)371-375
Number of pages5
JournalJournal of Ocular Pharmacology and Therapeutics
Volume32
Issue number6
DOIs
StatePublished - Jul 1 2016

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Hydrogen Sulfide
Intraocular Pressure
Rabbits
latanoprost
Cysteine
Hyperemia
New Zealand
Reading
Gases
GYY 4137

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology

Cite this

Effect of hydrogen sulfide donors on intraocular pressure in rabbits. / Salvi, Ankita; Bankhele, Pratik; Jamil, Jamal; Chitnis, Madhura Kulkarni; Njie-Mbye, Ya Fatou; Ohia, Sunny E.; Opere, Catherine A.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 32, No. 6, 01.07.2016, p. 371-375.

Research output: Contribution to journalArticle

Salvi, A, Bankhele, P, Jamil, J, Chitnis, MK, Njie-Mbye, YF, Ohia, SE & Opere, CA 2016, 'Effect of hydrogen sulfide donors on intraocular pressure in rabbits', Journal of Ocular Pharmacology and Therapeutics, vol. 32, no. 6, pp. 371-375. https://doi.org/10.1089/jop.2015.0144
Salvi, Ankita ; Bankhele, Pratik ; Jamil, Jamal ; Chitnis, Madhura Kulkarni ; Njie-Mbye, Ya Fatou ; Ohia, Sunny E. ; Opere, Catherine A. / Effect of hydrogen sulfide donors on intraocular pressure in rabbits. In: Journal of Ocular Pharmacology and Therapeutics. 2016 ; Vol. 32, No. 6. pp. 371-375.
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abstract = "Purpose: In this study, we investigated the effect of a slow-releasing hydrogen sulfide (H2S) donor, GYY 4137, on intraocular pressure (IOP) in normotensive rabbits. Furthermore, we compared the IOP-lowering action of GYY 4137 with those elicited by other H2S-producing compounds, l-cysteine and ACS67 (a hybrid compound of latanoprost with an H2S-releasing moiety). Methods: IOP was measured in New Zealand normotensive male albino rabbits using a pneumatonometer (model 30 classic; Reichert Ophthalmic Instruments, Depew, NY). At 0 h, 50 μL of test compounds were applied topically to 1 eye of each animal, while the contralateral eye received the same quantity of vehicle (saline). IOP was measured hourly until baseline IOP readings were attained and animal eyes monitored for potential side effects (i.e., tearing, hyperemia). Results: GYY 4137 (0.1{\%}-2{\%}) produced a dose-dependent decrease in IOP reaching a maximum of 27.8{\%} ± 3.14{\%} (n = 5) after 6 h. Interestingly, a significant contralateral effect was observed in vehicle-treated controls eyes at all doses tested. l-cysteine (5{\%}) and ACS67 (0.005{\%}) also elicited a significant (P <0.01) decrease in IOP that achieved a maximum of 28.84{\%} ± 1.53{\%} (n = 5) and 23.27{\%} ± 0.51{\%} (n = 5), respectively, after 3 h. All 3 H2S-producing compounds also caused a significant contralateral effect in vehicle-treated control eyes. Conclusion: We conclude that GYY 4137 and other H2S-producing donors can reduce IOP in normotensive rabbits. However, the profile of IOP-lowering action of GYY 4137 was different from the other H2S donors affirming its ability to act as a slow-releasing gas donor.",
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AU - Bankhele, Pratik

AU - Jamil, Jamal

AU - Chitnis, Madhura Kulkarni

AU - Njie-Mbye, Ya Fatou

AU - Ohia, Sunny E.

AU - Opere, Catherine A.

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