Effect of hydrogen sulfide on cyclic AMP production in isolated bovine and porcine neural retinae

Ya Fatou Njie-Mbye, Odelia Y N Bongmba, Chinwe C. Onyema, Abhishek Chitnis, Madhura Kulkarni, Catherine A. Opere, Angela M. Leday, Sunny E. Ohia

Research output: Contribution to journalArticle

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Abstract

Hydrogen sulfide (H2S) has been reported to exert pharmacological effects on neural and non-neural tissues from several mammalian species. In the present study, we examined the role of the intracellular messenger, cyclic AMP in retinal response to H2S donors, sodium hydrosulfide (NaHS) and sodium sulfide (Na2S) in cows and pigs. Isolated bovine and porcine neural retinae were incubated in oxygenated Krebs buffer solution prior to exposure to varying concentrations of NaHS, Na 2S or the diterpene activator of adenylate cyclase, forskolin. After incubation at different time intervals, tissue homogenates were prepared for cyclic AMP assay using a well established methodology. In isolated bovine and porcine retinae, the combination of both phosphodiesterase inhibitor, IBMX (2 mM) and forskolin (10 μM) produced a synergistic increase (P <0.001) in cyclic AMP concentrations over basal levels. NaHS (10 nM-100 μM) produced a time-dependent increase in cyclic AMP concentrations over basal levels which reached a maximum at 20 min in both bovine and porcine retinae. At this time point, both NaHS and Na2S (10 nM-100 μM) caused a significant (P <0.05) dose-dependent increase in cyclic AMP levels in bovine and porcine retinae. For instance, NaHS (100 nM) elicited a four-fold and three-fold increase in cyclic AMP concentrations in bovine and porcine retinae respectively whilst higher concentrations of Na2S (100 μM) produced a much lesser effect in both species. In bovine and porcine retinae, the effects caused by forskolin (10 μM) on cyclic AMP production were not potentiated by addition of low or high concentrations of both NaHS and Na2S. We conclude that H2S donors can increase cyclic AMP production in isolated neural retinae from cows and pigs. Bovine retina appears to be more sensitive to the stimulatory effect of H2S donors on cyclic nucleotide production than its porcine counterpart indicating that species differences exist in the magnitude of this response. Furthermore, effects produced by forskolin on cyclic AMP formation were not additive with those elicited by H2S donors suggesting that these agents may share a common mechanism in their action on the adenylyl cyclase pathway.

Original languageEnglish
Pages (from-to)487-494
Number of pages8
JournalNeurochemical Research
Volume35
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Hydrogen Sulfide
Cyclic AMP
Retina
Swine
Colforsin
Adenylyl Cyclases
Tissue
1-Methyl-3-isobutylxanthine
Phosphodiesterase Inhibitors
Diterpenes
Cyclic Nucleotides
sodium bisulfide
Assays
Buffers
sodium sulfide
Pharmacology

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Biochemistry

Cite this

Njie-Mbye, Y. F., Bongmba, O. Y. N., Onyema, C. C., Chitnis, A., Kulkarni, M., Opere, C. A., ... Ohia, S. E. (2010). Effect of hydrogen sulfide on cyclic AMP production in isolated bovine and porcine neural retinae. Neurochemical Research, 35(3), 487-494. https://doi.org/10.1007/s11064-009-0085-7

Effect of hydrogen sulfide on cyclic AMP production in isolated bovine and porcine neural retinae. / Njie-Mbye, Ya Fatou; Bongmba, Odelia Y N; Onyema, Chinwe C.; Chitnis, Abhishek; Kulkarni, Madhura; Opere, Catherine A.; Leday, Angela M.; Ohia, Sunny E.

In: Neurochemical Research, Vol. 35, No. 3, 03.2010, p. 487-494.

Research output: Contribution to journalArticle

Njie-Mbye, YF, Bongmba, OYN, Onyema, CC, Chitnis, A, Kulkarni, M, Opere, CA, Leday, AM & Ohia, SE 2010, 'Effect of hydrogen sulfide on cyclic AMP production in isolated bovine and porcine neural retinae', Neurochemical Research, vol. 35, no. 3, pp. 487-494. https://doi.org/10.1007/s11064-009-0085-7
Njie-Mbye, Ya Fatou ; Bongmba, Odelia Y N ; Onyema, Chinwe C. ; Chitnis, Abhishek ; Kulkarni, Madhura ; Opere, Catherine A. ; Leday, Angela M. ; Ohia, Sunny E. / Effect of hydrogen sulfide on cyclic AMP production in isolated bovine and porcine neural retinae. In: Neurochemical Research. 2010 ; Vol. 35, No. 3. pp. 487-494.
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