Effect of inhibition of cyclooxygenase on pre- and postjunctional actions of peroxides in the iris-ciliary body

Jennifer Graham, S. Olubusayo Awe, Angela M. LeDay, Justin C. Rice, Gretel Ramos, Lydia C. Harris, Catherine A. Opere, Sunny E. Ohia

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Abstract

In the present study, we investigated the effect of inhibition of cyclooxygenase (COX) with flurbiprofen (FBF) on peroxide-induced enhancement of field-stimulated [3H]norepinephrine ([3H]NE) release from bovine isolated irides. Furthermore, the effect of FBF was examined on peroxide- induced attenuation of contractions evoked by carbachol on this tissue. Irides were prepared for studies of neurotransmitter release and for measurement of contractile tension in vitro. Pretreatment of tissues with FBF (10 μM) caused significant (P <0.001) rightward shifts of concentration- response curves to H2O2 and also decreased cumene hydroperoxide (cuOOH)- induced enhancement of evoked [3H]NE release. FBF (10 μM) partially prevented the attenuation of carbachol-induced contractions induced by H2O2 (300 μM) and cuOOH (300 μM). We conclude that inhibition of the biosynthesis of prostanoids reduced both the prejunctional stimulatory effects of H2O2 and cuOOH on sympathetic neurotransmission and inhibitory effects of peroxides on carbachol-induced contractions the in the bovine isolated iris.

Original languageEnglish
Pages (from-to)519-525
Number of pages7
JournalNeurochemical Research
Volume25
Issue number4
DOIs
Publication statusPublished - 2000

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry

Cite this

Graham, J., Awe, S. O., LeDay, A. M., Rice, J. C., Ramos, G., Harris, L. C., ... Ohia, S. E. (2000). Effect of inhibition of cyclooxygenase on pre- and postjunctional actions of peroxides in the iris-ciliary body. Neurochemical Research, 25(4), 519-525. https://doi.org/10.1023/A:1007568227387