Effect of interleukin-4 on the synthesis of the third component of complement by pulmonary epithelial cells

Karlyle K. Christian-Ritter, Laura D. Hill, Eric B. Hoie, Terence L. Zach

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Complement activation in the lung is important in a variety of physiological and pathological conditions. The third component of complement, C3, is the pivotal constituent of the complement cascade. C3 is produced in the lung by several cell types including pulmonary epithelial cells. Because pulmonary epithelial cells and T lymphocytes may interact within the lung to regulate local immune responses, we examined the effect of a T lymphocyte- derived cytokine, interleukin-4 (IL-4) on C3 production by A549 human pulmonary epithelial cells. Treatment of A549 cells with IL-4 increased C3 production in a time- and dose-dependent fashion. Concentrations of IL-4 ≥ 0.1 ng/ml significantly increased C3 production. Maximal increase in C3 synthesis occurred after stimulation of A549 cells with IL-4 (10 ng/ml) for 3 days. Preincubation of IL-4 with a neutralizing anti-human IL-4 antibody inhibited IL-4's effect on C3 production. The relative abundance of C3 messenger RNA levels in A549 cells increased following IL-4 treatment, indicating that IL-4's effects on C3 production were pretranslational. Intercellular communication between T lymphocytes and pulmonary epithelial cells via cytokines such as IL-4 may be important in the local regulation of C3 gene expression during the inflammatory response.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalAmerican Journal of Pathology
Volume144
Issue number1
StatePublished - Jan 1994
Externally publishedYes

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Interleukin-4
Epithelial Cells
Lung
T-Lymphocytes
Cytokines
Complement C3
Complement Activation
Gene Expression Regulation
Interleukin-10
Messenger RNA
Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Effect of interleukin-4 on the synthesis of the third component of complement by pulmonary epithelial cells. / Christian-Ritter, Karlyle K.; Hill, Laura D.; Hoie, Eric B.; Zach, Terence L.

In: American Journal of Pathology, Vol. 144, No. 1, 01.1994, p. 171-176.

Research output: Contribution to journalArticle

Christian-Ritter, Karlyle K. ; Hill, Laura D. ; Hoie, Eric B. ; Zach, Terence L. / Effect of interleukin-4 on the synthesis of the third component of complement by pulmonary epithelial cells. In: American Journal of Pathology. 1994 ; Vol. 144, No. 1. pp. 171-176.
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